Relation of Retinal Oxygen Measures to Electrophysiology and Survival Indicators after Permanent, Incomplete Ischemia in Rats.
Relation of Retinal Oxygen Measures to Electrophysiology and Survival Indicators after Permanent, Incomplete Ischemia in Rats.
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DOI：
10.1007/s12975-020-00799-9
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发表时间：
2020-12
影响因子：
6.9
通讯作者：
Shahidi M
中科院分区：
文献类型：
--
作者：
Matei N;Leahy S;Auvazian S;Thomas B;Blair NP;Shahidi M
在永久性双侧颈总动脉闭塞（BCCAO）的实验性缺血模型中的研究已经报道了视网膜电生理功能降低，伴有视网膜内变性和神经胶质增生。在目前的研究中，我们测试的假设，长期（长达14天）BCCAO损害氧输送（DO2），影响氧代谢（MO2）和提取分数（OEF），电生理功能，形态和生化途径。21只大鼠接受BCCAO（N=12）或假手术（N=9），并在3天、7天或14天后在不同的组中进行评价。对双眼进行视网膜电图（ERG）、光学相干断层扫描（OCT）、血流和血管氧分压成像、形态学和生化评价。在BCCAO后3、7和14天报告了ERG b波振幅降低和隐式时间延迟。所有BCCAO组的总视网膜血流量、MO2和DO2均减少。3天和7天组的OEF均增加，而14天组的OEF与假手术组相比无显著差异。BCCAO后14天，视网膜总层和内层厚度减少，而外核层厚度和胶质增生增加。BCCAO后3天，含有片段化DNA的细胞核增加。BCCAO后OEF的代偿性升高不能满足组织需求，导致随后的MO2降低。在持续性缺血后遗症中，视网膜MO2、DO2和视网膜功能之间的相关性被证明是显著的。总之，DO2、MO2和OEF的测量可用于表征威胁视力的病理中的可挽救组织。
Studies in experimental ischemia models by permanent bilateral common carotid artery occlusion (BCCAO) have reported reduced retinal electrophysiological function, coupled with inner retinal degeneration and gliosis. In the current study, we tested the hypothesis that long-term (up to 14 days) BCCAO impairs oxygen delivery (DO2), which affects oxygen metabolism (MO2) and extraction fraction (OEF), electrophysiological function, morphology, and biochemical pathways. Twenty-one rats underwent BCCAO (N=12) or sham surgery (N=9) and were evaluated in separate groups after 3-, 7-, or 14-days. Electroretinography (ERG), optical coherence tomography, blood flow and vascular oxygen tension imaging, morphological and biochemical evaluations were performed in both eyes. Reduced ERG b-wave amplitudes and delayed implicit times were reported at 3-, 7-, and 14-days following BCCAO. Total retinal blood flow, MO2, and DO2 were reduced in all BCCAO groups. OEF was increased in both 3- and 7-day groups, while no significant difference was observed in OEF at 14-days compared to the sham group. At 14-days following BCCAO, total and inner retinal layer thickness were reduced, while the outer nuclear layer thickness and gliosis were increased. There was an increase in nuclei containing fragmented DNA at 3-days following BCCAO. The compensatory elevation in OEF following BCCAO did not meet the tissue demand, resulting in the subsequent reduction of MO2. The associations between retinal MO2, DO2, and retinal function were shown to be significant in the sequalae of persistent ischemia. In sum, measurements of DO2, MO2, and OEF may become useful for characterizing salvageable tissue in vision threatening pathologies.
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影响因子：
17.8
作者：
Kur, Joanna;Newman, Eric A.;Chan-Ling, Tailoi
通讯作者：
Chan-Ling, Tailoi
影响因子：
5.5
作者：
Krogh, A
通讯作者：
Krogh, A
影响因子：
3.4
作者：
BARNETT, NL;OSBORNE, NN
通讯作者：
OSBORNE, NN
影响因子：
1.9
作者：
HOOD, DC;BIRCH, DG
通讯作者：
BIRCH, DG
影响因子：
3.7
作者：
Joachim, Stephanie C.;Renner, Marina;Dick, H. Burkhard
通讯作者：
Dick, H. Burkhard