Structure and mechanism of the alkane-oxidizing enzyme AlkB.
Structure and mechanism of the alkane-oxidizing enzyme AlkB.
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烷烃氧化酶AlkB的结构和作用机理。
DOI：
10.1038/s41467-023-37869-z
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发表时间：
2023-04-17
影响因子：
16.6
通讯作者：
Feng L
中科院分区：
文献类型：
--
作者：
Guo X;Zhang J;Han L;Lee J;Williams SC;Forsberg A;Xu Y;Austin RN;Feng L
Alkanes are the most energy-rich form of carbon and are widely dispersed in the environment. Their transformation by microbes represents a key step in the global carbon cycle. Alkane monooxygenase (AlkB), a membrane-spanning metalloenzyme, converts straight chain alkanes to alcohols in the first step of the microbially-mediated degradation of alkanes, thereby playing a critical role in the global cycling of carbon and the bioremediation of oil. AlkB biodiversity is attributed to its ability to oxidize alkanes of various chain lengths, while individual AlkBs target a relatively narrow range. Mechanisms of substrate selectivity and catalytic activity remain elusive. Here we report the cryo-EM structure of AlkB, which provides a distinct architecture for membrane enzymes. Our structure and functional studies reveal an unexpected diiron center configuration and identify molecular determinants for substrate selectivity. These findings provide insight into the catalytic mechanism of AlkB and shed light on its function in alkane-degrading microorganisms. Alkane monooxygenase (AlkB) plays a key role in the global carbon cycle and remediation of oil spills. Here, the authors report the cryo-EM structure of AlkB to provide insight into the catalytic mechanism and substrate selectivity.
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影响因子：
5.2
作者：
Bertrand EM;Keddis R;Groves JT;Vetriani C;Austin RN
通讯作者：
Austin RN
影响因子：
64.8
作者：
Jumper J;Evans R;Pritzel A;Green T;Figurnov M;Ronneberger O;Tunyasuvunakool K;Bates R;Žídek A;Potapenko A;Bridgland A;Meyer C;Kohl SAA;Ballard AJ;Cowie A;Romera-Paredes B;Nikolov S;Jain R;Adler J;Back T;Petersen S;Reiman D;Clancy E;Zielinski M;Steinegger M;Pacholska M;Berghammer T;Bodenstein S;Silver D;Vinyals O;Senior AW;Kavukcuoglu K;Kohli P;Hassabis D
通讯作者：
Hassabis D
DOI：
10.1107/s0907444904019158
发表时间：
2004-12-01
影响因子：
2.2
作者：
Emsley, P;Cowtan, K
通讯作者：
Cowtan, K
影响因子：
16.6
作者：
Austin, Rachel N.;Luddy, Kate;Groves, John T.
通讯作者：
Groves, John T.
影响因子：
64.8
作者：
Bai Y;McCoy JG;Levin EJ;Sobrado P;Rajashankar KR;Fox BG;Zhou M
通讯作者：
Zhou M