Proteomic analysis of non-heading Chinese cabbage infected with Hyaloperonospora parasitica.
Proteomic analysis of non-heading Chinese cabbage infected with Hyaloperonospora parasitica.
复制标题
感染寄生大膜孢菌的不结球大白菜的蛋白质组学分析。
DOI：
10.1016/j.jprot.2013.11.028
复制
发表时间：
2014
影响因子：
3.3
通讯作者：
Ying Li
中科院分区：
文献类型：
--
作者：
Cheng;Li Wang;Die Hu;Ali RamuliMaquina Riquicho;Tongkun Liu;X. Hou;Ying Li
霜霉病是不结球白菜上的一种严重的真菌病害，由侵染十字花科植物的寄生透明顶孢霉引起。为了进行育种改良，研究人员必须了解不结球白菜抗击H。寄生虫感染。利用2-DE蛋白质组分析技术，比较了不结球白菜幼苗感染白菜的叶片蛋白质组。只在感染后不同时间点用水处理的寄生虫。通过MS分析，确定了91个丰度差异显著的蛋白质点(>；n 2倍，p<；n 0.05)。接种了寄生虫的叶子。其次，提出了一种针对不相容相互作用的抵抗策略。这个网络由几个功能成分组成，包括促进乙烯的生物合成和能量供应，平衡ROS的产生和清除，加速蛋白质代谢和光呼吸，降低光合作用，并诱导光系统修复。这些发现增加了我们对植物与病原菌不亲和互作的认识，也为植物分子过程的功能提供了新的见解，这将有助于发现新的病原菌控制策略。本研究报告了利用2-DE和MS对不结球白菜与霜霉病不亲和互作的蛋白质组学分析。这些蛋白质被划分为不同的功能类别，如氨基酸和碳水化合物代谢、光合作用和光呼吸、蛋白质代谢、信号转导、氧化还原动态平衡和乙烯生物合成。同时，几个关键蛋白被确定与乙烯信号、ROS清除和抗性相关蛋白相关。与这些结果一致的是，接种后乙烯生物合成基因和反应基因的表达以及抗氧化酶的活性都增加了。这些发现为进一步了解植物抗性的分子机制提供了新的见解。
Downy mildew is a serious fungal disease in non-heading Chinese cabbage (Brassica campestrisssp.chinensisMakino) that is caused byHyaloperonospora parasitica, which infects members of the Brassicaceae family. For breeding improvement, researchers must understand the defence mechanisms employed by non-heading Chinese cabbage to combatH. parasiticainfection. Using 2-DE protein analysis, we compared the proteomes from leaves of non-heading Chinese cabbage seedlings that were infected withH. parasiticaor that were only treated with water at different time points post-infection. By MS analysis, 91 protein spots with significant differences in abundance (> 2-fold,p< 0.05) were identified in mock- andH. parasitica-inoculated leaves. Next, a resistance strategy for incompatible interactions was proposed. This network consisted of several functional components, including enhanced ethylene biosynthesis and energy supply, balanced ROS production and scavenging, accelerated protein metabolism and photorespiratory, reduced photosynthesis, and induced photosystem repair. These findings increase our knowledge of incompatible interactions between plants and pathogens and also provide new insight regarding the function of plant molecular processes, which should assist in the discovery of new strategies for pathogen control.Biological significanceThis study reported the proteomic analysis of the incompatible interactions between non-heading Chinese cabbage and downy mildew using 2-DE and MS. In total, 91 protein spots that were related to the resistance response were identified. These proteins were assigned to different functional categories, such as amino acid and carbohydrate metabolism, photosynthesis and photorespiration, protein metabolism, signal transduction, redox homeostasis, and ethylene biosynthesis. Meanwhile, several key proteins were determined to be associated with ethylene signalling, ROS scavenging and resistance-related proteins. Consistent with these results, the expression of ethylene biosynthesis genes and response genes, as well as the activity of antioxidant enzymes, increased after inoculation. These findings provide new insight for further understanding the molecular mechanisms of plant resistance.
影响因子：
7.4
作者：
Circu, Magdalena L.;Aw, Tak Yee
通讯作者：
Aw, Tak Yee
影响因子：
56.9
作者：
BECKMANN, RP;MIZZEN, LA;WELCH, WJ
通讯作者：
WELCH, WJ