Whole-transcriptome sequencing and ceRNA interaction network of temporomandibular joint osteoarthritis.
Whole-transcriptome sequencing and ceRNA interaction network of temporomandibular joint osteoarthritis.
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颞下颌关节骨关节炎的全转录组测序和ceRNA相互作用网络
DOI：
10.3389/fgene.2022.962574
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发表时间：
2022
影响因子：
3.7
通讯作者：
Wu, Gaoyi
中科院分区：
文献类型：
--
作者：
Wu, Fan;An, Yanxin;Zhou, Libo;Zhao, Yuqing;Chen, Lei;Wang, Jing;Wu, Gaoyi
关键词：
目的：探讨非编码RNA(NcRNAs)在颞下颌关节骨关节炎(TMJOA)中的潜在生物学功能。方法：采用全转录组测序的方法，对TMJOA患者和正常对照组的差异表达基因(Degs)进行分析。用Metascape软件分析DEGS的功能和途径，并用Cytoscape软件构建了竞争内源RNA(Cerna)网络。结果：共检测到137个DEmRNAs、65个DEmiRNAs、132个DElncRNAs和29个DECircRNAs。对DEmRNAs的功能注释表明，免疫反应和细胞凋亡与TMJOA密切相关，并提出了与TMJOA相关的关键信号通路，包括慢性抑郁和PPAR信号通路。我们鉴定了重要的mRNAs，包括Klrk1、ADIPOQ、CryAB和Hspa1b。值得注意的是，关节软骨中ADIPOQ的表达与正常组相比显著上调(10倍，p<；0.001)。通过对受Cerna网络调控的DEmRNAs的功能分析，我们发现ncRNAs参与了自噬和细胞凋亡的调节。此外，还验证了显著的DEncRNAs(lncRNA-COX7A1、lncRNA-cHTOP、lncRNA-UFM1、ciRNA166和CircRNA1531)，其中，CircRNA1531(14.5倍，p；0.001)和lncRNA-cHTOP(14.8倍，p&t；0.001)是最显著下调的ncRNAs。结论：本研究显示了lncRNAs、CircRNAs、miRNAs和mRNAs作为临床生物标志物的潜力，并为了解它们在TMJOA中的作用提供了转录水平的见解。本研究确定了与免疫和凋亡相关的mRNAs的转录特征，以及与自噬和细胞凋亡相关的ncRNAs的特征，为深入了解TMJOA中的ncRNAs提供了依据。
Purpose: The aim of this study was to conduct a comprehensive transcriptomic analysis to explore the potential biological functions of noncoding RNA (ncRNAs) in temporomandibular joint osteoarthritis (TMJOA). Methods: Whole transcriptome sequencing was performed to identify differentially expressed genes (DEGs) profiles between the TMJOA and normal groups. The functions and pathways of the DEGs were analyzed using Metascape, and a competitive endogenous RNA (ceRNA) network was constructed using Cytoscape software. Results: A total of 137 DEmRNAs, 65 DEmiRNAs, 132 DElncRNAs, and 29 DEcircRNAs were identified between the TMJOA and normal groups. Functional annotation of the DEmRNAs revealed that immune response and apoptosis are closely related to TMJOA and also suggested key signaling pathways related to TMJOA, including chronic depression and PPAR signaling pathways. We identified vital mRNAs, including Klrk1, Adipoq, Cryab, and Hspa1b. Notably, Adipoq expression in cartilage was significantly upregulated in TMJOA compared with normal groups (10-fold, p < 0.001). According to the functional analysis of DEmRNAs regulated by the ceRNA network, we found that ncRNAs are involved in the regulation of autophagy and apoptosis. In addition, significantly DEncRNAs (lncRNA-COX7A1, lncRNA-CHTOP, lncRNA-UFM1, ciRNA166 and circRNA1531) were verified, and among these, circRNA1531 (14.5-fold, p < 0.001) and lncRNA-CHTOP (14.8-fold, p < 0.001) were the most significantly downregulated ncRNAs. Conclusion: This study showed the potential of lncRNAs, circRNAs, miRNAs, and mRNAs may as clinical biomarkers and provides transcriptomic insights into their functional roles in TMJOA. This study identified the transcriptomic signatures of mRNAs associated with immunity and apoptosis and the signatures of ncRNAs associated with autophagy and apoptosis and provides insight into ncRNAs in TMJOA.
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基于生物信息学分析的类风湿关节炎和骨关节炎基因特征筛选
影响因子：
3.4
作者：
He P;Zhang Z;Liao W;Xu D;Fu M;Kang Y
通讯作者：
Kang Y
DOI：
10.1016/j.clim.2012.12.011
发表时间：
2013-03
期刊：
Clinical immunology (Orlando, Fla.)
影响因子：
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作者：
Haseeb A;Haqqi TM
通讯作者：
Haqqi TM
影响因子：
12.3
作者：
Marchese FP;Raimondi I;Huarte M
通讯作者：
Huarte M
影响因子：
5.6
作者：
Musumeci G;Castrogiovanni P;Trovato FM;Weinberg AM;Al-Wasiyah MK;Alqahtani MH;Mobasheri A
通讯作者：
Mobasheri A
DOI：
10.1111/febs.12005
发表时间：
2012-11
期刊：
The FEBS journal
影响因子：
--
作者：
Oh DH;Rigas D;Cho A;Chan JY
通讯作者：
Chan JY