Evidence for a hydrogenosomal-type anaerobic ATP generation pathway in Acanthamoeba castellanii.
Evidence for a hydrogenosomal-type anaerobic ATP generation pathway in Acanthamoeba castellanii.
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DOI：
10.1371/journal.pone.0069532
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Roger AJ
中科院分区：
文献类型：
--
作者：
Leger MM;Gawryluk RM;Gray MW;Roger AJ
不同的、远缘相关的真核细胞谱系已经适应了低氧环境，并且具有通过氧化磷酸化失去产生三磷酸腺苷（ATP）的能力的线粒体相关细胞器。这些细胞器的一个子集，氢化酶体，已经获得了一套典型的ATP生成酶，通常在厌氧细菌中发现。这些酶的接受者在获得它们之前不可能存活下来，如果它仍然拥有祖先的电子传递链的话。在现代氢化酶体与线粒体的分化过程中，一定存在一个过渡性的细胞器，它同时具有电子传递链和无氧ATP生成途径。在这里，我们报告了一个现代的模拟器，这个细胞器的习惯性好氧的机会致病菌，卡氏阿米巴。这种生物体具有一套完整的酶，包括氢酶体样ATP生成途径，其中每一种都被预测为靶向线粒体。我们已经通过实验证实了线粒体定位的关键组成部分，这一途径使用串联质谱。这一证据是第一个支持的本地化和蛋白质组数据的一个电子传递链和氢酶体的能量代谢酶。我们的工作提供了对现代氢化酶体出现过程中可能发生的第一步的深入了解。
Diverse, distantly-related eukaryotic lineages have adapted to low-oxygen environments, and possess mitochondrion-related organelles that have lost the capacity to generate adenosine triphosphate (ATP) through oxidative phosphorylation. A subset of these organelles, hydrogenosomes, has acquired a set of characteristic ATP generation enzymes commonly found in anaerobic bacteria. The recipient of these enzymes could not have survived prior to their acquisition had it not still possessed the electron transport chain present in the ancestral mitochondrion. In the divergence of modern hydrogenosomes from mitochondria, a transitional organelle must therefore have existed that possessed both an electron transport chain and an anaerobic ATP generation pathway. Here, we report a modern analog of this organelle in the habitually aerobic opportunistic pathogen, Acanthamoeba castellanii. This organism possesses a complete set of enzymes comprising a hydrogenosome-like ATP generation pathway, each of which is predicted to be targeted to mitochondria. We have experimentally confirmed the mitochondrial localizations of key components of this pathway using tandem mass spectrometry. This evidence is the first supported by localization and proteome data of a mitochondrion possessing both an electron transport chain and hydrogenosome-like energy metabolism enzymes. Our work provides insight into the first steps that might have occurred in the course of the emergence of modern hydrogenosomes.
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影响因子：
3.4
作者：
Gawryluk, Ryan M. R.;Gray, Michael W.
通讯作者：
Gray, Michael W.
影响因子：
64.8
作者：
Boxma, B;de Graaf, RM;Hackstein, JHP
通讯作者：
Hackstein, JHP
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作者：
Dyall, SD;Yan, WH;Johnson, PJ
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Johnson, PJ
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3.5
作者：
ELLIS, JE;WILLIAMS, R;LLOYD, D
通讯作者：
LLOYD, D
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作者：
Hackstein, JHP
通讯作者：
Hackstein, JHP