Surfactant Protein A Can Affect Macrophage Phagocytosis: An Important Pathogenic Mechanism of Otitis Media with Effusion
Surfactant Protein A Can Affect Macrophage Phagocytosis: An Important Pathogenic Mechanism of Otitis Media with Effusion
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表面活性蛋白A影响巨噬细胞吞噬作用：渗出性中耳炎的重要致病机制
DOI：
10.1007/s10162-023-00893-3
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发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Li Li
中科院分区：
文献类型：
--
作者：
Tao Chen;Guodong Li;Wenwen Liu;Z. Fan;Li Li
渗出性中耳炎（OME），也称为分泌性中耳炎，是耳鼻喉科的常见疾病。主要表现为中耳积液和传导性听力损失。近年来，OME的病因学研究日益受到重视，其中免疫功能紊乱是OME的重要致病机制之一。然而，目前尚不清楚表面活性蛋白A（SPA）分泌的变化是否影响咽鼓管中巨噬细胞的吞噬活性，从而改变病原体清除，在发病过程中的OME。本研究建立了OME动物模型，并对其进行了评价。分析实验组和对照组咽鼓管灌洗液中SPA水平的差异。细胞实验表明，SPA可降低RAW264.7细胞CD64和SYK的表达，抑制RAW264.7细胞的吞噬功能。通过使用流式细胞术和免疫荧光，我们证实了巨噬细胞吞噬功能随着SPA水平的增加而降低。结论SPA影响巨噬细胞功能，在OME的发生发展中起一定作用。
Otitis media with effusion (OME), also known as secretory otitis media, is a common condition in otorhinolaryngology. The main manifestations include middle ear effusion and conductive hearing loss. Recently, increasing attention has been paid to the etiology of OME, wherein immune dysfunction is one important pathogenic mechanism. However, it is unknown whether changes in surfactant protein A (SPA) secretion affect the phagocytic activity of macrophages in the Eustachian tube, thereby altering pathogen clearance, during the pathogenesis of OME. In our study, an OME animal model was established and evaluated. Differences in SPA levels in Eustachian tube lavage fluid between the experimental and control groups were analyzed. Cell-based experiments revealed that SPA decreased the expression of CD64 and SYK and inhibited phagocytosis by RAW264.7 cells. By using flow cytometry and immunofluorescence, we confirmed that macrophage phagocytosis decreased with increasing SPA levels. Finally, we concluded that SPA affects macrophage function and plays a role in the occurrence and development of OME.
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DOI：
10.1164/rccm.200711-1661oc
发表时间：
2008-07-15
影响因子：
24.7
作者：
Janssen, William J.;McPhillips, Kathleen A.;Gardai, Shyra J.
通讯作者：
Gardai, Shyra J.
影响因子：
3.2
作者：
Abdel-Razek, Osama;Ni, Lan;Wang, Guirong
通讯作者：
Wang, Guirong
DOI：
10.1001/archotol.1987.01860020030007
发表时间：
1987
期刊：
Archives of otolaryngology--head & neck surgery
影响因子：
--
作者：
Bakaletz,LO;DeMaria,TF;Lim,DJ
通讯作者：
Lim,DJ
渗出性中耳炎的免疫学方面：淋巴细胞和巨噬细胞反应性的特征。
DOI：
10.1093/infdis/145.6.804
发表时间：
1982
期刊：
The Journal of infectious diseases
影响因子：
--
作者：
Yamanaka,T;Bernstein,JB;Cumella,J;Parker,C;Ogra,PL
通讯作者：
Ogra,PL