Virulence analysis of Staphylococcus aureus in a rabbit model of infected full-thickness wound under negative pressure wound therapy.
Virulence analysis of Staphylococcus aureus in a rabbit model of infected full-thickness wound under negative pressure wound therapy.
复制标题
负压创面治疗兔全层感染感染模型金黄色葡萄球菌毒力分析
DOI：
10.1007/s10482-017-0938-z
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发表时间：
2018-03
期刊：
影响因子：
--
通讯作者：
Tang P
中科院分区：
文献类型：
--
作者：
Liu D;Li Z;Wang G;Li T;Zhang L;Tang P
本研究旨在使用标准无菌纱布和负压伤口治疗（NPWT）在对照动物研究中评价金黄色葡萄球菌的毒力，包括agr的激活、基因表达和毒力因子的产生以及细菌侵袭深度。在第0天（细菌接种后6小时）、第2天、第4天、第6天和第8天在伤口床中心收获组织样本。激光扫描共聚焦显微镜进行，以获得生物发光图像，用于测量细菌入侵的深度。金黄色葡萄球菌的agrA表达和Eap、Spa、α毒素等毒力因子的转录和产生存在显著差异。在负压伤口治疗的作用下，细菌侵入深度明显减少。群体感应系统的显著不同的激活使得细胞与细胞之间的通信和许多定殖因子和毒力因子的调节能够在不同的微环境中随着时间的推移导致不同的基因表达和致病性。因此，agr系统代表了一个基本的监管模式，可以包括不同的适应性策略，并容纳水平获得的毒力决定因素。
The aim of this study was to evaluate the virulence of Staphylococcus aureus in a controlled animal study using the standard sterile gauze and negative pressure wound therapy (NPWT), including activation of agr, gene expression and production of virulence foctors and depth of bacterial invasion. The tissue specimens were harvested on days 0 (6 h after bacterial inoculation), 2, 4, 6, and 8 at the center of wound beds. Laser scanning confocal microscopy was performed to obtain bioluminescent images which were used to measure the depth of bacterial invasion. The agrA expression of S.aureus and the transcription and production of virulence factors including Eap, Spa and α-toxin were significantly different. The bacterial invasion depth was significantly less with effect of NPWT. The markedly different activation of quorum sensing systems that enable cell-to-cell communication and regulation of numerous colonization and virulence factors result in distinct gene expression and pathogenicity over time in different microenvironment. Thus, the agr system represents a fundamental regulatory paradigm that can encompass different adaptive strategies and accommodate horizontally acquired virulence determinants.
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影响因子：
4.6
作者：
Fleck, TM;Fleck, M;Grabenwoger, M
通讯作者：
Grabenwoger, M
影响因子：
2.9
作者：
Gurjala, Anandev N.;Geringer, Matthew R.;Mustoe, Thomas A.
通讯作者：
Mustoe, Thomas A.
影响因子：
4.1
作者：
Kreikemeyer, B;McDevitt, D;Podbielski, A
通讯作者：
Podbielski, A
影响因子：
3.1
作者：
MCGAVIN, MH;KRAJEWSKAPIETRASIK, D;HOOK, M
通讯作者：
HOOK, M
影响因子：
--
作者：
Fleischmann, W;Russ, M;Stampehl, M
通讯作者：
Stampehl, M