Genetic basis of orange spot formation in the guppy (Poecilia reticulata).
Genetic basis of orange spot formation in the guppy (Poecilia reticulata).
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DOI：
10.1186/s12862-021-01942-2
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发表时间：
2021-11-25
影响因子：
2.2
通讯作者：
Kawata M
中科院分区：
文献类型：
--
作者：
Kawamoto M;Ishii Y;Kawata M
为了理解雌性对彩色雄性纹饰的配偶选择的进化意义，必须了解这种纹饰的潜在调节机制，以研究纹饰如何与增加后代适应性或性吸引力的“雄性品质”相关联。孔雀鱼（Poecilia reticulata）是一种已建立的性选择研究模型系统，雌性更喜欢拥有更大、饱和度更高的橙色斑点的雄性作为潜在的伴侣。虽然以前的研究已经发现了一些与橙色斑形成相关的染色体区域和基因，但这些遗传元件在橙色斑形成中的调控和参与尚未阐明。本研究利用RNA-seq技术，研究了桔斑特异性基因的表达模式和某些颜色发育阶段，揭示了桔斑形成的遗传基础。将同一个体有橙色斑点的雄性孔雀鱼皮肤（橙色皮肤）与无颜色斑点的雄性孔雀鱼皮肤（暗色皮肤）的基因表达水平进行比较，鉴定出1102个差异表达基因（deg），其中橙色皮肤中上调基因630个，下调基因472个。此外，比较了三个发育阶段全干皮肤的基因表达水平，根据颜色发育鉴定出2247个基因为deg。这些分析表明，黄细胞的次生分化可能影响橙斑的形成。结果表明，橘黄色斑点的形成可能是由Csf1和甲状腺激素信号通路诱导的黄嘌呤细胞二次分化而非新生形成的。此外，我们提出了与橙色斑点的面积和饱和度相关的候选基因，这两个基因都被认为对女性的配偶选择很重要，并且独立调控。本研究提供了对橙斑形成的遗传和细胞调控机制的见解，这将有助于阐明这些过程是如何进化地维持与性选择相关的观赏性状的。在线版本包含补充材料，可在10.1186/s12862-021-01942-2获得。
To understand the evolutionary significance of female mate choice for colorful male ornamentation, the underlying regulatory mechanisms of such ornamentation must be understood for examining how the ornaments are associated with “male qualities” that increase the fitness or sexual attractiveness of offspring. In the guppy (Poecilia reticulata), an established model system for research on sexual selection, females prefer males possessing larger and more highly saturated orange spots as potential mates. Although previous studies have identified some chromosome regions and genes associated with orange spot formation, the regulation and involvement of these genetic elements in orange spot formation have not been elucidated. In this study, the expression patterns of genes specific to orange spots and certain color developmental stages were investigated using RNA-seq to reveal the genetic basis of orange spot formation. Comparing the gene expression levels of male guppy skin with orange spots (orange skin) with those without any color spots (dull skin) from the same individuals identified 1102 differentially expressed genes (DEGs), including 630 upregulated genes and 472 downregulated genes in the orange skin. Additionally, the gene expression levels of the whole trunk skin were compared among the three developmental stages and 2247 genes were identified as DEGs according to color development. These analyses indicated that secondary differentiation of xanthophores may affect orange spot formation. The results suggested that orange spots might be formed by secondary differentiation, rather than de novo generation, of xanthophores, which is induced by Csf1 and thyroid hormone signaling pathways. Furthermore, we suggested candidate genes associated with the areas and saturation levels of orange spots, which are both believed to be important for female mate choice and independently regulated. This study provides insights into the genetic and cellular regulatory mechanisms underlying orange spot formation, which would help to elucidate how these processes are evolutionarily maintained as ornamental traits relevant to sexual selection. The online version contains supplementary material available at 10.1186/s12862-021-01942-2.
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影响因子：
3.3
作者：
Braasch I;Brunet F;Volff JN;Schartl M
通讯作者：
Schartl M
影响因子：
3.3
作者：
Fraser BA;Whiting JR;Paris JR;Weadick CJ;Parsons PJ;Charlesworth D;Bergero R;Bemm F;Hoffmann M;Kottler VA;Liu C;Dreyer C;Weigel D
通讯作者：
Weigel D
影响因子：
56.9
作者：
BAGNARA, JT;MATSUMOTO, J;TAYLOR, JD
通讯作者：
TAYLOR, JD
影响因子：
3.8
作者：
HOUDE, AE
通讯作者：
HOUDE, AE
影响因子：
4.3
作者：
Dooley, Christopher M.;Schwarz, Heinz;Geisler, Robert
通讯作者：
Geisler, Robert