A portable system for processing donated whole blood into high quality components without centrifugation.
A portable system for processing donated whole blood into high quality components without centrifugation.
复制标题
一种用于加工的便携式系统将全血捐赠成高质量的成分而无需离心。
DOI：
10.1371/journal.pone.0190827
复制
发表时间：
2018
期刊：
影响因子：
3.7
通讯作者：
Shevkoplyas SS
中科院分区：
文献类型：
--
作者：
Gifford SC;Strachan BC;Xia H;Vörös E;Torabian K;Tomasino TA;Griffin GD;Lichtiger B;Aung FM;Shevkoplyas SS
The use of centrifugation-based approaches for processing donated blood into components is routine in the industrialized world, as disparate storage conditions require the rapid separation of ‘whole blood’ into distinct red blood cell (RBC), platelet, and plasma products. However, the logistical complications and potential cellular damage associated with centrifugation/apheresis manufacturing of blood products are well documented. The objective of this study was to evaluate a proof-of-concept system for whole blood processing, which does not employ electromechanical parts, is easily portable, and can be operated immediately after donation with minimal human labor. In a split-unit study (n = 6), full (~500mL) units of freshly-donated whole blood were divided, with one half processed by conventional centrifugation techniques and the other with the new blood separation system. Each of these processes took 2–3 hours to complete and were performed in parallel. Blood products generated by the two approaches were compared using an extensive panel of cellular and plasma quality metrics. Comparison of nearly all RBC parameters showed no significant differences between the two approaches, although the portable system generated RBC units with a slight but statistically significant improvement in 2,3-diphosphoglyceric acid concentration (p < 0.05). More notably, several markers of platelet damage were significantly and meaningfully higher in products generated with conventional centrifugation: the increase in platelet activation (assessed via P-selectin expression in platelets before and after blood processing) was nearly 4-fold higher for platelet units produced via centrifugation, and the release of pro-inflammatory mediators (soluble CD40-ligand, thromboxane B2) was significantly higher for centrifuged platelets as well (p < 0.01). This study demonstrated that a simple, passive system for separating donated blood into components may be a viable alternative to centrifugation—particularly for applications in remote or resource-limited settings, or for patients requiring highly functional platelet product.
登录
查看更多内容
从孕妇的外周血中分离出核红细胞（NRBC）的微流体方法。
影响因子：
3
作者：
Huang, R.;Barber, T. A.;Schmidt, M. A.;Tompkins, R. G.;Toner, M.;Bianchi, D. W.;Kapur, R.;Flejter, W. L.
通讯作者：
Flejter, W. L.
影响因子：
39.2
作者：
Kaufman, Richard M.;Djulbegovic, Benjamin;Tobian, Aaron A. R.
通讯作者：
Tobian, Aaron A. R.
影响因子：
7.5
作者：
Ghandforoush, Parviz;Sen, Tarun K.
通讯作者：
Sen, Tarun K.
影响因子：
2.9
作者：
Levin, Elena;Culibrk, Brankica;Devine, Dana V.
通讯作者：
Devine, Dana V.
影响因子：
2.9
作者：
Leytin, V;Allen, DJ;Freedman, J
通讯作者：
Freedman, J