A microfluidics approach for the isolation of nucleated red blood cells (NRBCs) from the peripheral blood of pregnant women.
A microfluidics approach for the isolation of nucleated red blood cells (NRBCs) from the peripheral blood of pregnant women.
复制标题
从孕妇的外周血中分离出核红细胞（NRBC）的微流体方法。
DOI：
10.1002/pd.2079
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发表时间：
2008-10
影响因子：
3
通讯作者：
Flejter, W. L.
中科院分区：
文献类型：
--
作者：
Huang, R.;Barber, T. A.;Schmidt, M. A.;Tompkins, R. G.;Toner, M.;Bianchi, D. W.;Kapur, R.;Flejter, W. L.
有核红细胞（NRBC）已在母体循环中被鉴定，并可能为监测和诊断母体、胎儿和新生儿的健康和疾病提供资源。过去用于分离和富集NRBC的策略限于导致低回收率和低于最佳细胞纯度的复杂方法。在这里，我们报告了一种高通量和高效的微流体装置的发展，用于分离罕见的NRBC从母体血液。使用微流控过程从58名孕妇的外周血中分离NRBC，该微流控过程由用于基于大小的细胞分离的微流控芯片和用于基于血红蛋白的细胞分离的磁性装置组成。微流体-磁性组合以非常高的效率（约99.99%）去除非目标红细胞和白色血细胞。该设备成功识别了58/58份终止前样本的外周血中的NRBC，平均值为37.44 NRBC/mL（范围为0.37-274.36 NRBC/mL）。这些结果与以前的研究进行了比较。微流体装置导致NRBC的富集超过先前描述的方法约10至20倍。分离的可靠性和NRBC产品的纯度有可能使随后的分子诊断测定的应用。
Nucleated red blood cells (NRBCs) have been identified in maternal circulation and potentially provide a resource for the monitoring and diagnosis of maternal, fetal, and neonatal health and disease. Past strategies used to isolate and enrich for NRBCs are limited to complex approaches that result in low recovery and less than optimal cell purity. Here we report the development of a high-throughput and highly efficient microfluidic device for isolating rare NRBCs from maternal blood. NRBCs were isolated from the peripheral blood of 58 pregnant women using a microfluidic process that consists of a microfluidic chip for size-based cell separation and a magnetic device for hemoglobin-based cell isolation. The microfluidic–magnetic combination removes nontarget red blood cells and white blood cells at a very high efficiency (∼99.99%). The device successfully identified NRBCs from the peripheral blood of 58/58 pretermination samples with a mean of 37.44 NRBC/mL (range 0.37–274.36 NRBC/mL). These results were compared with those from previous studies. The microfluidic device results in an approximate 10- to 20-fold enrichment of NRBCs over methods described previously. The reliability of isolation and the purity of the NRBC product have the potential to enable the subsequent application of molecular diagnostic assays.
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影响因子：
3
作者：
Bianchi, DW;Simpson, JL;Wapner, RJ
通讯作者：
Wapner, RJ
影响因子：
56.9
作者：
Huang, LR;Cox, EC;Sturm, JC
通讯作者：
Sturm, JC
影响因子：
3.2
作者：
Babochkina, T;Mergenthaler, S;Hahn, S
通讯作者：
Hahn, S
影响因子：
3
作者：
Kwon, K. H.;Jeon, Y. J.;Pang, M. G.
通讯作者：
Pang, M. G.
DOI：
10.1016/0002-9378(91)90024-l
发表时间：
1991-12-01
影响因子：
9.8
作者：
PRICE, JO;ELIAS, S;SIMPSON, JL
通讯作者：
SIMPSON, JL