Overexpression of Plasma Membrane H(+)-ATPase in Guard Cells Enhances Light-Induced Stomatal Opening, Photosynthesis, and Plant Growth in Hybrid Aspen.
Overexpression of Plasma Membrane H(+)-ATPase in Guard Cells Enhances Light-Induced Stomatal Opening, Photosynthesis, and Plant Growth in Hybrid Aspen.
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DOI：
10.3389/fpls.2021.766037
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发表时间：
2021
影响因子：
5.6
通讯作者：
Kinoshita T
中科院分区：
文献类型：
--
作者：
Toh S;Takata N;Ando E;Toda Y;Wang Y;Hayashi Y;Mitsuda N;Nagano S;Taniguchi T;Kinoshita T
Stomata in the plant epidermis open in response to light and regulate CO2 uptake for photosynthesis and transpiration for uptake of water and nutrients from roots. Light-induced stomatal opening is mediated by activation of the plasma membrane (PM) H+-ATPase in guard cells. Overexpression of PM H+-ATPase in guard cells promotes light-induced stomatal opening, enhancing photosynthesis and growth in Arabidopsis thaliana. In this study, transgenic hybrid aspens overexpressing Arabidopsis PM H+-ATPase (AHA2) in guard cells under the strong guard cell promoter Arabidopsis GC1 (AtGC1) showed enhanced light-induced stomatal opening, photosynthesis, and growth. First, we confirmed that AtGC1 induces GUS expression specifically in guard cells in hybrid aspens. Thus, we produced AtGC1::AHA2 transgenic hybrid aspens and confirmed expression of AHA2 in AtGC1::AHA2 transgenic plants. In addition, AtGC1::AHA2 transgenic plants showed a higher PM H+-ATPase protein level in guard cells. Analysis using a gas exchange system revealed that transpiration and the photosynthetic rate were significantly increased in AtGC1::AHA2 transgenic aspen plants. AtGC1::AHA2 transgenic plants showed a>20% higher stem elongation rate than the wild type (WT). Therefore, overexpression of PM H+-ATPase in guard cells promotes the growth of perennial woody plants.
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DOI：
10.1093/bioinformatics/8.3.275
发表时间：
1992-06-01
期刊：
COMPUTER APPLICATIONS IN THE BIOSCIENCES
影响因子：
--
作者：
JONES, DT;TAYLOR, WR;THORNTON, JM
通讯作者：
THORNTON, JM
影响因子：
4.6
作者：
Hayashi M;Inoue SI;Ueno Y;Kinoshita T
通讯作者：
Kinoshita T
DOI：
10.1126/science.1244454
发表时间：
2014-01-24
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Haruta M;Sabat G;Stecker K;Minkoff BB;Sussman MR
通讯作者：
Sussman MR
影响因子：
2.3
作者：
CONDON, AG;RICHARDS, RA;FARQUHAR, GD
通讯作者：
FARQUHAR, GD
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y