Does altered protein metabolism interfere with postmortem degradation analysis for PMI estimation?
Does altered protein metabolism interfere with postmortem degradation analysis for PMI estimation?
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DOI：
10.1007/s00414-018-1814-8
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发表时间：
2018-09
影响因子：
2.1
通讯作者：
Pittner S
中科院分区：
文献类型：
--
作者：
Zissler A;Ehrenfellner B;Foditsch EE;Monticelli FC;Pittner S
准确估计死后间隔 (PMI) 是法医常规工作的核心内容。最近，提出了一种基于死后肌肉蛋白降解分析的新方法。然而，在将该方法合理地应用于各种法医案件之前，仍有许多问题需要解答。为了评估体内蛋白质代谢的改变是否会干扰死后的降解模式，我们进行了一项比较研究。我们在大鼠中开发了标准化的动物退化模型，并从肌肉损伤中恢复的动物和诱发脊髓损伤后出现废用性肌肉萎缩的大鼠中收集了额外的肌肉样本。所有样品均通过 SDS-PAGE 和蛋白质印迹进行分析，标记特征良好的肌肉蛋白。发现原肌球蛋白在整个 PMI 调查过程中保持稳定，并且在再生和萎缩肌肉中未检测到任何变化。相比之下，结蛋白和纽蛋白带模式发生了显着的可预测的死后变化。虽然在废用性肌肉萎缩的死亡样本中没有检测到与天然模式的显着偏差，但有趣的是，从肌肉损伤中恢复的大鼠样本显示出额外的结蛋白和纽蛋白降解带，无论 PMI 如何，这些降解带在任何检查的死后样本中都不会以这种形式出现。体内代谢改变是否影响死后降解动力学，或者这些肌肉样本是否以常规方式经历死后降解，仍有待研究。
An accurate estimation of the postmortem interval (PMI) is a central aspect in forensic routine. Recently, a novel approach based on the analysis of postmortem muscle protein degradation has been proposed. However, a number of questions remain to be answered until sensible application of this method to a broad variety of forensic cases is possible. To evaluate whether altered in vivo protein metabolism interferes with postmortem degradation patterns, we conducted a comparative study. We developed a standardized animal degradation model in rats, and collected additional muscle samples from animals recovering from muscle injury and from rats with developed disuse muscle atrophy after induced spinal cord injury. All samples were analyzed by SDS-PAGE and Western blot, labeling well-characterized muscle proteins. Tropomyosin was found to be stable throughout the investigated PMI and no alterations were detected in regenerating and atrophic muscles. In contrast, significant predictable postmortem changes occurred in desmin and vinculin protein band patterns. While no significant deviations from native patterns were detected in at-death samples of disuse muscle atrophy, interestingly, samples of rats recovering from muscle injury revealed additional desmin and vinculin degradation bands that did not occur in this form in any of the examined postmortem samples regardless of PMI. It remains to be investigated whether in vivo-altered metabolism influences postmortem degradation kinetics or if such muscle samples undergo postmortem degradation in a regular fashion.
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影响因子：
2.1
作者：
Ehrenfellner B;Zissler A;Steinbacher P;Monticelli FC;Pittner S
通讯作者：
Pittner S
影响因子：
7.3
作者：
de Matos-Neto EM;Lima JD;de Pereira WO;Figuerêdo RG;Riccardi DM;Radloff K;das Neves RX;Camargo RG;Maximiano LF;Tokeshi F;Otoch JP;Goldszmid R;Câmara NO;Trinchieri G;de Alcântara PS;Seelaender M
通讯作者：
Seelaender M
DOI：
10.1016/j.biocel.2013.06.011
发表时间：
2013-10
影响因子：
4
作者：
Bodine, Sue C.
通讯作者：
Bodine, Sue C.
影响因子：
7.1
作者：
Ji, J. -R.;Takahashi, K.
通讯作者：
Takahashi, K.
影响因子：
2.1
作者：
Fucci, Nadia;Campobasso, Carlo P.;Pascali, V. L.
通讯作者：
Pascali, V. L.