Effects of Lipolysis-Stimulated Lipoprotein Receptor on Tight Junctions of Pancreatic Ductal Epithelial Cells in Hypertriglyceridemic Acute Pancreatitis.
Effects of Lipolysis-Stimulated Lipoprotein Receptor on Tight Junctions of Pancreatic Ductal Epithelial Cells in Hypertriglyceridemic Acute Pancreatitis.
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脂解刺激的脂蛋白受体对高甘油末术急性急性胰腺炎胰腺导管细胞紧密连接的影响。
DOI：
10.1155/2022/4234186
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发表时间：
2022
影响因子：
--
通讯作者：
Huang, Jiean
中科院分区：
文献类型：
--
作者：
Wang, Jie;Qin, Mengbin;Wu, Qing;Yang, Huiying;Wei, Biwei;Xie, Jinlian;Qin, Yingying;Liang, Zhihai;Huang, Jiean
We investigated the effects of lipolysis-stimulated lipoprotein receptor (LSR) on the tight junctions (TJs) of pancreatic ductal epithelial cells (PDECs) in hypertriglyceridemic acute pancreatitis (HTGAP). Sprague-Dawley rats were fed standard rat chow or a high-fat diet and injected with sodium taurocholate to obtain normal and HTGAP rats, respectively. Serum triglyceride (TG) levels, pathological changes, TJ proteins in the pancreas, and TJ ultrastructure of PDECs were assessed. LSR overexpression (OE) and knockdown (KD) HPDE6-C7 models were designed and cultured in a high-fat environment. Protein levels were quantified by Western blotting. Cell monolayer permeability was detected using FITC-Dextran. Serum TG concentration and pancreatic scores were higher in the HTGAP group than in the normal group. Among the TJ proteins, LSR protein expression was significantly lower in the HTGAP group than in the acute pancreatitis (AP) group. Tricellulin (TRIC) expression in the pancreatic ductal epithelia was higher in the HTGAP group than in the AP group. The HTGAP group had lower TJ protein levels, wider intercellular space, and widespread cellular necrosis with disappearance of cell junction structures. In the cell study, TJ proteins were downregulated and the cellular barrier was impaired by palmitic acid (PA), which was reversed by LSR-OE, whereas LSR-KD downregulated the TJ proteins and aggravated PA-induced cellular barrier impairment. Hypertriglyceridemia downregulates the TJ proteins in PDECs, which may impair the pancreatic ductal mucosal barrier function. LSR regulation can change the effects of HTG on cellular barrier function by upregulating the TJ proteins.
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影响因子：
21.1
作者：
Cai J;Culley MK;Zhao Y;Zhao J
通讯作者：
Zhao J
DOI：
10.1083/jcb.200510043
发表时间：
2005-12-19
期刊：
The Journal of cell biology
影响因子：
--
作者：
Ikenouchi J;Furuse M;Furuse K;Sasaki H;Tsukita S;Tsukita S
通讯作者：
Tsukita S
影响因子：
6.6
作者：
Cording, Jimmi;Guenther, Ramona;Blasig, Ingolf E.
通讯作者：
Blasig, Ingolf E.
DOI：
10.1158/1541-7786.mcr-16-0085-t
发表时间：
2017-02
期刊：
Molecular cancer research : MCR
影响因子：
--
作者：
Reaves DK;Hoadley KA;Fagan-Solis KD;Jima DD;Bereman M;Thorpe L;Hicks J;McDonald D;Troester MA;Perou CM;Fleming JM
通讯作者：
Fleming JM
影响因子：
3.3
作者：
Higashi T;Miller AL
通讯作者：
Miller AL