In Vivo Evaluation of Different Collagen Scaffolds in an Achilles Tendon Defect Model.
In Vivo Evaluation of Different Collagen Scaffolds in an Achilles Tendon Defect Model.
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DOI：
10.1155/2018/6432742
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发表时间：
2018
影响因子：
--
通讯作者：
Tischer T
中科院分区：
文献类型：
--
作者：
Gabler C;Saß JO;Gierschner S;Lindner T;Bader R;Tischer T
在本研究中，在跟腱缺损模型中对新引入的牛交联胶原支架（试验材料）进行了体内研究，并与市售猪胶原支架（对照材料）进行了比较。总共检查了28只雄性Sprague道利大鼠（约400 g）。用一种支架材料替代右后肢5 mm的确定跟腱缺损。人道处死后，横切后肢进行试验。通过拉伸试验进行生物力学评价（每组n = 8，观察时间：28天）。双侧未手术肌腱用作对照（自体肌腱，n = 4）。对于组织学评价，在术后14天和28天处死12只动物（每组和时间点n = 3）。根据细胞的存在和细胞迁移到支架中以及支架的结构和降解，定性评价染色的切片（苏木精和伊红）。在测试前使用MRI额外分析所有横断后肢，以验证使用胶原支架的肌腱修复在观察期后是否仍然完整。两种支架材料的最大失效载荷（试验材料：54.5 ± 16.4 N，对照材料：63.1 ± 19.5 N）均在自体肌腱范围内（76.6 ± 11.6 N，p ≥ 0.07）。与使用两种支架修复的肌腱相比，天然肌腱的刚度高两倍（p ≤ 0.01），撕裂强度高约五倍（p ≤ 0.01）。组织学结果表明，试验材料和对照材料均未诱导炎症，但试验材料经历了较慢的重塑过程。通过MRI观察到总体修复失败率为48%。与市售对照材料相比，新开发的试验材料的实验数据显示出相似的结果。高修复失败率表明，建议将MRI作为验证实验数据的辅助测量工具。
In the present study, a newly introduced bovine cross-linked collagen scaffold (test material) was investigated in vivo in an Achilles tendon defect model and compared to a commercially available porcine collagen scaffold (control material). In total, 28 male Sprague Dawley rats (about 400 g) were examined. The defined Achilles tendon defect of 5 mm of the right hind limb was replaced by one of the scaffold materials. After euthanasia, the hind limbs were transected for testing. Biomechanical evaluation was carried out via tensile testing (n = 8 each group, observation time: 28 days). Nonoperated tendons from the bilateral side were used as a control (native tendon, n = 4). For the histological evaluation, 12 animals were sacrificed at 14 and 28 days postoperatively (n = 3 each group and time point). Stained slices (Hematoxylin & Eosin) were evaluated qualitatively in terms of presence of cells and cell migration into scaffolds as well as structure and degradation of the scaffold. All transected hind limbs were additionally analyzed using MRI before testing to verify if the tendon repair using a collagen scaffold was still intact after the observation period. The maximum failure loads of both scaffold materials (test material: 54.5 ± 16.4 N, control: 63.1 ± 19.5 N) were in the range of native tendon (76.6 ± 11.6 N, p ≥ 0.07). The stiffness of native tendons was twofold higher (p ≤ 0.01) and the tear strength was approximately fivefold higher (p ≤ 0.01) compared to the repaired tendons with both scaffolds. Histological findings indicated that neither the test nor the control material induced inflammation, but the test material underwent a slower remodeling process. An overall repair failure rate of 48% was observed via MRI. The experimental data of the newly developed test material showed similar outcomes compared to the commercially available control material. The high repair failure rate indicated that MRI is recommended as an auxiliary measurement tool to validate experimental data.
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影响因子：
4.1
作者：
Finni, T.;Bernabei, M.;Maas, H.
通讯作者：
Maas, H.
影响因子：
3
作者：
Choi, Sungwook;Kim, Myung Ku;Kang, Hyunseong
通讯作者：
Kang, Hyunseong
影响因子：
0.3
作者：
Clark, Randy R.;Dierckman, Brian D.;Burns, Joseph P.
通讯作者：
Burns, Joseph P.
影响因子：
0.6
作者：
Gungormus, Cansin;Cetinkaya, Mehmet Alper;Demirutku, Alper
通讯作者：
Demirutku, Alper
DOI：
10.1016/j.arthro.2011.06.038
发表时间：
2012-01-01
影响因子：
4.7
作者：
Barber, F. Alan;Burns, Joseph P.;Litchfield, Robert B.
通讯作者：
Litchfield, Robert B.