Activity-based proteomic and metabolomic approaches for understanding metabolism.
Activity-based proteomic and metabolomic approaches for understanding metabolism.
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基于活动的蛋白质组学和代谢组学方法，用于理解代谢。
DOI：
10.1016/j.copbio.2014.02.001
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发表时间：
2014-08
影响因子：
7.7
通讯作者：
Nomura, Daniel K.
中科院分区：
文献类型：
--
作者：
Hunerdosse, Devon;Nomura, Daniel K.
越来越多的人类疾病与代谢改变有关，包括肥胖、糖尿病、动脉粥样硬化、癌症和神经退行性疾病。人们对新陈代谢的关注大多集中在人们熟知的新陈代谢途径上，而在很大程度上忽视了在(病理性)生理环境中运行的大多数生化途径，部分原因是大量未确定特征和未被发现的代谢途径。为应对这一挑战而出现的一种技术是基于活性的蛋白质图谱(ABPP)，它使用基于活性的化学探针来广泛评估特征酶和未特征酶的功能状态。这篇综述将集中在ABPP如何与抑制剂发现平台和功能代谢组技术相结合，导致发现扩大了我们对健康和疾病中的新陈代谢的知识。
There are an increasing number of human pathologies that have been associated with altered metabolism, including obesity, diabetes, atherosclerosis, cancer, and neurodegenerative diseases. Most attention on metabolism has been focused on well-understood metabolic pathways and has largely ignored most of the biochemical pathways that operate in (patho)physiological settings, in part because of the vast landscape of uncharacterized and undiscovered metabolic pathways. One technology that has arisen to meet this challenge is activity-based protein profiling (ABPP) that uses activity-based chemical probes to broadly assess the functional states of both characterized and uncharacterized enzymes. This review will focus on how ABPP, coupled with inhibitor discovery platforms and functional metabolomic technologies, have led to discoveries that have expanded our knowledge of metabolism in health and disease.
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影响因子：
64.8
作者：
通讯作者：
--
影响因子：
50.3
作者：
Figueroa ME;Abdel-Wahab O;Lu C;Ward PS;Patel J;Shih A;Li Y;Bhagwat N;Vasanthakumar A;Fernandez HF;Tallman MS;Sun Z;Wolniak K;Peeters JK;Liu W;Choe SE;Fantin VR;Paietta E;Löwenberg B;Licht JD;Godley LA;Delwel R;Valk PJ;Thompson CB;Levine RL;Melnick A
通讯作者：
Melnick A
DOI：
10.1124/jpet.111.180257
发表时间：
2011-07-01
影响因子：
3.5
作者：
Ahn, Kay;Smith, Sarah E.;Cravatt, Benjamin F.
通讯作者：
Cravatt, Benjamin F.
影响因子：
14.8
作者：
Blum, Galia;von Degenfeld, Georges;Bogyo, Matthew
通讯作者：
Bogyo, Matthew
影响因子：
14.8
作者：
Adibekian, Alexander;Martin, Brent R.;Wang, Chu;Hsu, Ku-Lung;Bachovchin, Daniel A.;Niessen, Sherry;Hoover, Heather;Cravatt, Benjamin F.
通讯作者：
Cravatt, Benjamin F.