TAT-mediated photochemical internalization results in cell killing by causing the release of calcium into the cytosol of cells.
TAT-mediated photochemical internalization results in cell killing by causing the release of calcium into the cytosol of cells.
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DOI：
10.1016/j.bbagen.2012.06.020
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发表时间：
2012-11
影响因子：
3
通讯作者：
Pellois, Jean-Philippe
中科院分区：
文献类型：
--
作者：
Muthukrishnan, Nandhini;Johnson, Gregory A.;Lim, Jongdoo;Simanek, Eric E.;Pellois, Jean-Philippe
内吞细胞器的裂解是许多细胞递送方法中的必要步骤。这在光化学内化方法中有效地实现，但伴随该过程的细胞死亡仍然是一个问题。我们研究了荧光肽TMR-TAT的光化学内化引起的细胞死亡机制。TMR-TAT在胞吞和光照射后杀死细胞。TMR-TAT对内吞细胞器的溶解导致胞质溶胶中钙浓度的快速增加。TMR-TAT与含有钙的内吞细胞器在照射之前共定位，并且光化学内化导致这些细胞器的内腔内容物的释放。钌红和环孢菌素A是线粒体中钙输入和线粒体渗透性转换孔的抑制剂，可抑制细胞死亡。TMR-TAT介导的光化学内化导致钙稳态破坏。线粒体中钙的随后输入是伴随光化学内化的细胞死亡的原因。了解内吞细胞器的裂解如何影响细胞生理学并导致细胞死亡对于开发最佳递送方法至关重要。
Lysis of endocytic organelles is a necessary step in many cellular delivery methodologies. This is achieved efficiently in the photochemical internalization approach but the cell death that accompanies this process remains a problem. We investigate the mechanisms of cell death that accompanies photochemical internalization of the fluorescent peptide TMR-TAT. TMR-TAT kills cells after endocytosis and light irradiation. The lysis of endocytic organelles by TMR-TAT causes a rapid increase in the concentration of calcium in the cytosol. TMR-TAT co-localizes with endocytic organelles containing calcium prior to irradiation and photochemical internalization leads to the release of the lumenal content of these organelles. Ruthenium red and cyclosporin A, inhibitors of calcium import in mitochondria and of the mitochondria permeability transition pore, inhibit cell death. TMR-TAT mediated photochemical internalization leads to a disruption of calcium homeostasis. The subsequent import of calcium in mitochondria is a causative factor of the cell death that accompanies photochemical internalization. Understanding how the lysis of endocytic organelles affects cellular physiology and causes cell death is crucial to the development of optimal delivery methodologies.
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影响因子：
64.8
作者：
ALLISON, AC;MAGNUS, IA;YOUNG, MR
通讯作者：
YOUNG, MR
DOI：
10.1074/jbc.m109.025353
发表时间：
2009-07-31
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Baumgartner HK;Gerasimenko JV;Thorne C;Ferdek P;Pozzan T;Tepikin AV;Petersen OH;Sutton R;Watson AJ;Gerasimenko OV
通讯作者：
Gerasimenko OV
影响因子：
4.9
作者：
Nascimento, Fabio D.;Sancey, Lucie;Hayashi, Mirian A. F.
通讯作者：
Hayashi, Mirian A. F.
DOI：
10.1016/0006-291x(90)91362-v
发表时间：
1990-08-31
影响因子：
3.1
作者：
LATTANZIO, FA
通讯作者：
LATTANZIO, FA
影响因子：
9.2
作者：
Gerasimenko, JV;Tepikin, AV;Gerasimenko, OV
通讯作者：
Gerasimenko, OV