Proteomic comparison of selective breeding and growth hormone transgenesis in fish: Unique pathways to enhanced growth.
Proteomic comparison of selective breeding and growth hormone transgenesis in fish: Unique pathways to enhanced growth.
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DOI：
10.1016/j.jprot.2018.08.013
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发表时间：
2019-02-10
影响因子：
3.3
通讯作者：
Macqueen DJ
中科院分区：
文献类型：
--
作者：
Causey DR;Kim JH;Stead DA;Martin SAM;Devlin RH;Macqueen DJ
在用于食品生产和科学研究的鱼类中，可以通过选择性繁殖或通过生长激素(GH)转基因(GHT)瞬时诱导实现快速生长。这些通往相似的更高表型的不同途径的蛋白质组学基础仍未确定，与健康参数相关的暗示也是如此。我们使用骨骼肌蛋白质组学在Coho鲑鱼(Oncorhynchus Kisutch)中解决了这一知识差距，假设i)选择性繁殖和GHT受到平行和独特的生长系统变化的支持，ii)快速生长的鱼类品种降低了为免疫功能分配资源的范围。在注射PBS(对照)或Poly I：C(模拟感染)后，对GHT和生长选择的菌株进行定量分析，并与野生型进行比较。我们确定了每个生长增强型菌株的肌肉蛋白质组的重塑，这是惊人的不重叠。GIGT的特点是驱动蛋白质合成的系统局部上调，而生长选择的鱼呈现出更大和更多样化的一套变化，与许多代谢和细胞途径的复杂变化一致。Poly I：C对肌肉蛋白质组无明显影响。这项研究表明，不同的蛋白质组图谱可以解释表面上相似的增强生长表型，提高了我们对人为动物品系生长机制的理解。这项工作首次对鲑鱼快速生长的不同人为途径的机制提供了蛋白质组学方面的见解。高通量蛋白质组图谱被用来揭示支持促进生长的变化，比较了转基因生长激素骨骼肌(GHT)和选择性繁殖的鲑鱼品系与它们的野生型对应品系。对比过去相同鱼类品系的mRNA水平比较，我们的数据揭示了GHT和选择性繁殖品系之间令人惊讶的实质性蛋白质组差异。这些发现表明，用于食品生产和科学研究的不同类型的鱼类品种的生长促进表型背后存在许多独特的分子机制。鱼类快速生长的机制基础鲜为人知。在高度促进生长的鱼类品系中进行的比较蛋白质组学分析。比较转基因和驯化的鱼类品系，增强生长的明显基础。高度不同的蛋白质组图谱可以解释表面上相似的生长表型。这项研究加强了对人为动物品系如何实现快速生长的理解。
In fish used for food production and scientific research, fast growth can be achieved via selective breeding or induced instantaneously via growth hormone (GH) transgenesis (GHT). The proteomic basis for these distinct routes towards a similar higher phenotype remains uncharacterized, as are associated implications for health parameters. We addressed this knowledge gap using skeletal muscle proteomics in coho salmon (Oncorhynchus kisutch), hypothesising that i) selective breeding and GHT are underpinned by both parallel and unique changes in growth systems, and ii) rapidly-growing fish strains have lowered scope to allocate resources towards immune function. Quantitative profiling of GHT and growth-selected strains was done in comparison to wild-type after injection with PBS (control) or Poly I:C (to mimic infection). We identified remodelling of the muscle proteome in each growth-enhanced strain that was strikingly non-overlapping. GHT was characterized by focal upregulation of systems driving protein synthesis, while the growth-selected fish presented a larger and more diverse set of changes, consistent with complex alterations to many metabolic and cellular pathways. Poly I:C had little detectable effect on the muscle proteome. This study demonstrates that distinct proteome profiles can explain outwardly similar enhanced growth phenotypes, improving our understanding of growth mechanisms in anthropogenic animal strains. This work provides the first proteomic insights into mechanisms underpinning different anthropogenic routes to rapid growth in salmon. High-throughput proteomic profiling was used to reveal changes supporting enhanced growth, comparing skeletal muscle of growth hormone transgenic (GHT) and selectively-bred salmon strains with their wild-type counterparts. Contrasting past mRNA-level comparisons of the same fish strains, our data reveals a surprisingly substantial proteomic divergence between the GHT and selectively bred strains. The findings demonstrate that many unique molecular mechanisms underlie growth-enhanced phenotypes in different types of fish strain used for food production and scientific research. Mechanistic basis for rapid growth poorly understood in fish. Comparative proteomic profiling done in fish strains showing highly enhanced growth. Distinct basis for enhanced growth comparing transgenic and domesticated fish strains. Highly distinct proteome profiles may explain outwardly similar growth phenotypes. Study enhances understanding of how rapid growth is achieved in anthropogenic animal strains.
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