Insulin-like growth factors stimulate chemotaxis in human melanoma cells.
Insulin-like growth factors stimulate chemotaxis in human melanoma cells.
复制标题
胰岛素样生长因子刺激人类黑色素瘤细胞的趋化性。
DOI：
10.1016/s0006-291x(88)81338-x
复制
发表时间：
1988
影响因子：
3.1
通讯作者：
Elliott Schiffmann
中科院分区：
文献类型：
--
作者：
M. Stracke;E. C. Kohn;S. Aznavoorian;Lori L. Wilson;David S. Salomon;H. C. Krutzsch;Lance A. Liotta;Elliott Schiffmann
胰岛素和胰岛素样生长因子刺激高转移性人黑色素瘤细胞系A2058的运动胰岛素样生长因子-I（IGF-I）是最有效的，与胰岛素和胰岛素样生长因子-II（IGF-II）的活性相比，在10 nM的浓度下具有最大响应，其峰值为300-400 nM。使用棋盘格分析，IGF-I和胰岛素的反应主要是趋化性，虽然胰岛素有显着的趋化动力学成分。百日咳毒素不抑制对任何这些多肽的应答。然而，在以前的研究中，这些相同的A2058细胞对自分泌运动因子的运动反应被百日咳毒素显著抑制。Scatchard分析表明其结合亲和力很高（Kd = 3 × 10− 10 M），估计每个细胞有5000个受体。这些研究表明，除了它们的促有丝分裂特性之外，某些生长因子还可以通过它们诱导运动的能力来显著增强肿瘤细胞的转移。
Insulin and insulin-like growth factors stimulate motility in the highly metastatic human melanoma cell line, A2058. Insulin-like growth factor-I (IGF-I) is the most potent with a maximal response at a concentration of 10 nM compared to the activities of insulin and insulin-like growth factor-II (IGF-II) which peak at 300–400 nM. Using checkerboard analysis, the responses to IGF-I and insulin are predominantly chemotactic, although insulin had a significant chemokinetic component. Pertussis toxin does not inhibit the response to any of these polypeptides. However, in previous studies, it was shown that the motile response to autocrine motility factor from these same A2058 cells was markedly inhibited by pertussis toxin.125I-labelled IGF-I binds saturably and specifically to the A2058 cells. Scatchard analysis indicates a high binding affinity (Kd ∼ 3 × 10−10M) and an estimated 5000 receptors/cell. These studies indicate that in addition to their mitogenic properties, certain growth factors may profoundly enhance metastasis of tumor cells by their ability to induce motility.
DOI：
10.1016/s0021-9258(18)34631-3
发表时间：
1982-05
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
J. Massagué;M. Czech
通讯作者：
J. Massagué;M. Czech
血小板衍生生长因子通过不同的结构决定因素刺激小鼠 3T3 细胞有丝分裂和白细胞趋化性。
DOI：
10.1172/jci111135
发表时间：
1983
期刊：
The Journal of clinical investigation
影响因子：
--
作者：
Williams,LT;Antoniades,HN;Goetzl,EJ
通讯作者：
Goetzl,EJ
猪胰岛素对人 T 淋巴细胞的体外趋化活性。
DOI：
--
发表时间：
1987
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Berman,JS;Center,DM
通讯作者：
Center,DM