A sensitive enzymatic method (SK-013) for detection of Treponema denticola, Porphyromonas gingivals and Bacteroides forsythus in subgingival plaque samples.
A sensitive enzymatic method (SK-013) for detection of Treponema denticola, Porphyromonas gingivals and Bacteroides forsythus in subgingival plaque samples.
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一种灵敏的酶法 (SK-013)，用于检测龈下菌斑样本中的齿垢密螺旋体、牙龈卟啉单胞菌和福赛斯拟杆菌。
DOI：
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发表时间：
1992
影响因子：
3.5
通讯作者：
K. Okuda
中科院分区：
文献类型：
--
作者：
K. Seida;A. Saito;S. Yamada;K. Ishihara;Y. Naito;K. Okuda
建立了一种用于龈下菌斑样品中肽酶活性快速检测的酶法SK-013。结果表明，该方法对牙龈卟啉单胞菌、牙密螺旋体、连翘拟杆菌及部分嗜糖菌具有特异性。本研究的目的是确定SK-013是否可以指示牙周病细菌的存在，包括牙牙分枝杆菌、牙龈卟啉卟啉杆菌和连翘杆菌，这些细菌会产生胰蛋白酶样酶。从10个临床健康部位和30个牙周病变部位取龈下菌斑，取3个纸点。检测菌斑样品的SK-013活性，并采用免疫荧光技术对菌斑样品中牙齿菌、牙龈卟啉菌和连翘菌数量进行计数。在病变部位，SK-013活性与龈指数、菌斑指数、探诊深度、探诊出血等临床参数显著相关。这些生物的存在与SK-013活性之间存在显著的相关性。钉螺的存在与SK-013活性的相关系数高于与其他微生物的相关系数。结果表明，SK-013可作为牙龈下菌斑中齿牙霉、牙龈假单胞菌和连翘假单胞菌的细胞数量指标。
An enzymatic method, SK-013, was developed for rapid detection of the peptidase activity in subgingival plaque samples. This method was found to have specificity for Porphyromonas gingivalis, Treponema denticola, Bacteroides forsythus, and some Capnocytophaga strains. The purpose of this study was to determine whether SK-013 could indicate the presence of periodontopathic bacteria, including T. denticola, P. gingivalis and B. forsythus, which produce trypsin-like enzymes. Subgingival plaque samples were taken from 10 clinically healthy sites and 30 periodontally diseased sites with 3 paper points. SK-013 activity of plaque samples was assayed, and the numbers of T. denticola, P. gingivalis and B. forsythus in the sample were counted by immunofluorescence technique. In diseased sites, the SK-013 activity was significantly correlated with clinical parameters such as Gingival Index, Plaque Index, probing depth and bleeding on probing. A significant correlation was found between the presence of these organisms and SK-013 activity. Correlation coefficients between the presence of T. denticola and SK-013 activity were higher than those with other organisms. These findings indicate that the SK-013 is useful as an indicator of cell population of T. denticola, P. gingivalis and B. forsythus in subgingival plaque.
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影响因子：
--
作者：
Genco,RJ;Zambon,JJ;Christersson,LA
通讯作者：
Christersson,LA
伴放线放线杆菌的选择性培养基以及该微生物在幼年牙周炎中的发病率。
影响因子：
4.3
作者：
Mandell,RL;Socransky,SS
通讯作者：
Socransky,SS
早期幼年牙周炎患者的四环素治疗。
影响因子：
4.3
作者：
Novak,MJ;Polson,AM;Adair,SM
通讯作者：
Adair,SM
影响因子：
6.7
作者：
DZINK, JL;SOCRANSKY, SS;HAFFAJEE, AD
通讯作者：
HAFFAJEE, AD