Real time blood testing using quantitative phase imaging.
Real time blood testing using quantitative phase imaging.
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DOI：
10.1371/journal.pone.0055676
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Popescu G
中科院分区：
文献类型：
--
作者：
Pham HV;Bhaduri B;Tangella K;Best-Popescu C;Popescu G
我们展示了一个实时血液检测系统，可以提供远程诊断，在经济困难的地区最小的人为干预。我们的仪器结合了无标记光学成像与并行计算的新进展。具体来说，我们使用定量相位成像提取红细胞形态与纳米级的灵敏度和NVIDIA的CUDA编程语言进行真实的时间细胞水平的分析。当血液涂片通过焦点平移时，我们的系统能够以40帧/秒的速度分割和分析100万像素视场中的所有细胞。从每个细胞测量的各种诊断参数（例如，表面积、球度和最小圆柱直径）目前在现有技术的临床仪器中是不可用的。此外，我们表明，我们的仪器正确地恢复红细胞的体积分布，证明了良好的协议与正常患者和小红细胞和大红细胞贫血的细胞计数器的结果。由我们的仪器输出的最终数据表示与这些形态参数相关的数字阵列，而不是图像。因此，存储这些数据所需的存储器是兆字节量级的，这允许它们经由例如蜂窝网络进行远程传输。我们设想，这样的系统将大大增加血液检测的可及性，并可能为数字血液学铺平道路。
We demonstrate a real-time blood testing system that can provide remote diagnosis with minimal human intervention in economically challenged areas. Our instrument combines novel advances in label-free optical imaging with parallel computing. Specifically, we use quantitative phase imaging for extracting red blood cell morphology with nanoscale sensitivity and NVIDIA’s CUDA programming language to perform real time cellular-level analysis. While the blood smear is translated through focus, our system is able to segment and analyze all the cells in the one megapixel field of view, at a rate of 40 frames/s. The variety of diagnostic parameters measured from each cell (e.g., surface area, sphericity, and minimum cylindrical diameter) are currently not available with current state of the art clinical instruments. In addition, we show that our instrument correctly recovers the red blood cell volume distribution, as evidenced by the excellent agreement with the cell counter results obtained on normal patients and those with microcytic and macrocytic anemia. The final data outputted by our instrument represent arrays of numbers associated with these morphological parameters and not images. Thus, the memory necessary to store these data is of the order of kilobytes, which allows for their remote transmission via, for example, the cellular network. We envision that such a system will dramatically increase access for blood testing and furthermore, may pave the way to digital hematology.
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影响因子：
3.4
作者：
Mir M;Tangella K;Popescu G
通讯作者：
Popescu G
DOI：
10.1073/pnas.0804612105
发表时间：
2008-08-05
影响因子：
11.1
作者：
Cui, Xiquan;Lee, Lap Man;Yang, Changhuei
通讯作者：
Yang, Changhuei
影响因子：
3.7
作者：
HOSHEN, J;KOPELMAN, R
通讯作者：
KOPELMAN, R
影响因子：
48
作者：
Choi, Wonshik;Fang-Yen, Christopher;Feld, Michael S.
通讯作者：
Feld, Michael S.
影响因子：
3.8
作者：
Kalentev, Oleksandr;Rai, Abha;Schneider, Ralf
通讯作者：
Schneider, Ralf