Effect of suppression of arabinoxylan synthetic genes in wheat endosperm on chain length of arabinoxylan and extract viscosity.
Effect of suppression of arabinoxylan synthetic genes in wheat endosperm on chain length of arabinoxylan and extract viscosity.
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DOI：
10.1111/pbi.12361
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发表时间：
2016-01
影响因子：
13.8
通讯作者：
Mitchell RA
中科院分区：
文献类型：
--
作者：
Freeman J;Lovegrove A;Wilkinson MD;Saulnier L;Shewry PR;Mitchell RA
阿拉伯木聚糖（AX）是小麦（Triticum aestivum L.）胚乳细胞壁内的主要成分，占多糖的70%。小麦籽粒水提物的粘度是影响各种最终用途加工的关键性状，这在很大程度上取决于胚乳AX的性质。我们之前已经发现，通过下调与AX链延伸相关的TaGT43_2或TaGT47_2基因（分别与拟南芥中的IRX9和IRX10同源）和与3链阿拉伯糖单取代相关的TaXAT1基因，转基因小麦胚乳AX有显著影响。在这里，我们使用这些转基因系来研究可溶性和不溶性组分中AX的量之间的关系，用特征粘度测量的这些链长分布以及对提取物粘度的总体影响。在表达TaGT43_2或TaGT47_2 RNAi基因的转基因株系中，水萃取组分（WE - AX）和水不溶性碱萃取组分（AE - AX）的固有粘度比对照株系降低了10% - 50%。在TaXAT1 RNAi细胞系中，WE‐AX的特性粘度降低了15%，但对AE‐AX的特性粘度没有一致的影响。所有转基因系均表现出提取物黏度的降低，其中TaGT43_2和TaGT47_2 RNAi系的效果比TaXAT1 RNAi系的效果大（降幅达6倍）。这些影响可以用WE - AX的数量和链长减少来解释，其中数量减少的影响更大。因此，通过抑制单基因靶点可以大大降低小麦籽粒提取物的粘度。
Arabinoxylan (AX) is the dominant component within wheat (Triticum aestivum L.) endosperm cell walls, accounting for 70% of the polysaccharide. The viscosity of aqueous extracts from wheat grain is a key trait influencing the processing for various end uses, and this is largely determined by the properties of endosperm AX. We have previously shown dramatic effects on endosperm AX in transgenic wheat by down‐regulating either TaGT43_2 or TaGT47_2 genes (orthologues to IRX9 and IRX10 in Arabidopsis, respectively) implicated in AX chain extension and the TaXAT1 gene responsible for monosubstitution by 3‐linked arabinose. Here, we use these transgenic lines to investigate the relationship between amounts of AX in soluble and insoluble fractions, the chain‐length distribution of these measured by intrinsic viscosity and the overall effect on extract viscosity. In transgenic lines expressing either the TaGT43_2 or TaGT47_2 RNAi transgenes, the intrinsic viscosities of water‐extractable (WE‐AX) and of a water‐insoluble alkaline‐extracted fraction (AE‐AX) were decreased by between 10% and 50% compared to control lines. In TaXAT1 RNAi lines, there was a 15% decrease in intrinsic viscosity of WE‐AX but no consistent effect on that of AE‐AX. All transgenic lines showed decreases in extract viscosity with larger effects in TaGT43_2 and TaGT47_2 RNAi lines (by up to sixfold) than in TaXAT1 RNAi lines (by twofold). These effects were explained by the decreases in amount and chain length of WE‐AX, with decreases in amount having the greater influence. Extract viscosity from wheat grain can therefore be greatly decreased by suppression of single gene targets.
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影响因子：
7.4
作者：
Pellny, Till K.;Lovegrove, Alison;Mitchell, Rowan A. C.
通讯作者：
Mitchell, Rowan A. C.
影响因子：
2.4
作者：
Finnie, S. M.;Bettge, A. D.;Morris, C. F.
通讯作者：
Morris, C. F.
影响因子：
3.8
作者：
GRUPPEN, H;HAMER, RJ;VORAGEN, AGJ
通讯作者：
VORAGEN, AGJ
影响因子：
3.8
作者：
SAULNIER, L;PENEAU, N;THIBAULT, JF
通讯作者：
THIBAULT, JF
影响因子：
5.6
作者：
Burton RA;Fincher GB
通讯作者：
Fincher GB