Cellular Localization and Regulation of Expression of the PLET1 Gene in Porcine Placenta.
Cellular Localization and Regulation of Expression of the PLET1 Gene in Porcine Placenta.
复制标题
DOI：
10.3390/ijms17122048
复制
发表时间：
2016-12-07
影响因子：
5.6
通讯作者：
Yu M
中科院分区：
文献类型：
--
作者：
Teng L;Hong L;Liu R;Chen R;Li X;Yu M
胎盘表达转录本1（PLET 1）基因在猪和小鼠胎盘中表达，已发现其在小鼠滋养层细胞命运决定中具有潜在作用。本研究的结果表明，猪PLET 1 mRNA和蛋白质仅在妊娠第15、26、50和95天的滋养层细胞中表达（猪的妊娠时间为114天），表明PLET 1可以作为猪滋养层细胞的有用标记物。此外，发现PLET 1蛋白随着妊娠进展从细胞质重新分布到滋养层细胞的顶侧，这表明PLET 1在建立稳定的滋养层和子宫内膜上皮层中的作用。另外，通过交替切割和多聚腺苷酸化（阿帕）产生了两个3′ UTR长度不同但编码相同蛋白质的转录本，且PLET 1-L转录本在猪胎盘中的表达随着妊娠的进行而显著上调。此外，我们还利用荧光素酶检测系统证实了miR-365- 3 p与PLET 1基因之间的相互作用。我们的研究结果表明PLET 1在猪胎盘发育中起重要作用。
The placenta expressed transcript 1 (PLET1) gene, which is expressed in placentas of pigs and mice, has been found to have a potential role in trophoblast cell fate decision in mice. Results of this study showed that the porcine PLET1 mRNA and protein were expressed exclusively in trophoblast cells on Days 15, 26, 50, and 95 of gestation (gestation length in the pig is 114 days), indicating that the PLET1 could be a useful marker for porcine trophoblast cells. Additionally, PLET1 protein was found to be redistributed from cytoplasm to the apical side of trophoblast cells as gestation progresses, which suggests a role of PLET1 in the establishment of a stable trophoblast and endometrial epithelial layers. In addition, two transcripts that differ in the 3′ UTR length but encode identical protein were identified to be generated by the alternative cleavage and polyadenylation (APA), and the expression of PLET1-L transcript was significantly upregulated in porcine placentas as gestation progresses. Furthermore, we demonstrated the interaction between the miR-365-3p and PLET1 gene using luciferase assay system. Our findings imply an important role of PLET1 in the placental development in pigs.
登录
查看更多内容
影响因子：
10.5
作者：
Lianoglou S;Garg V;Yang JL;Leslie CS;Mayr C
通讯作者：
Mayr C
影响因子：
3.7
作者：
Engelmann JC;Spang R
通讯作者：
Spang R
乙酰肝素酶的表达与约克夏猪和梅山猪胎盘折叠双层的品种特异性形态特征相关
影响因子：
3.6
作者：
Hong, Linjun;Hou, Chunyan;Yu, Mei
通讯作者：
Yu, Mei
影响因子：
3.6
作者：
Krawczynski, Kamil;Najmula, Joanna;Kaczmarek, Monika M.
通讯作者：
Kaczmarek, Monika M.
影响因子：
3.8
作者：
Chikh, K;Vey, S;Millat, G
通讯作者：
Millat, G