The Tip of the Iceberg: On the Roles of Regulatory Small RNAs in the Virulence of Enterohemorrhagic and Enteropathogenic Escherichia coli.
The Tip of the Iceberg: On the Roles of Regulatory Small RNAs in the Virulence of Enterohemorrhagic and Enteropathogenic Escherichia coli.
复制标题
DOI：
10.3389/fcimb.2016.00105
复制
发表时间：
2016
影响因子：
5.7
通讯作者：
El-Fenej J
中科院分区：
文献类型：
--
作者：
Bhatt S;Egan M;Jenkins V;Muche S;El-Fenej J
肠出血性和肠致病性大肠杆菌是胃肠道病原体，破坏肠道微绒毛，在感染细胞上形成附着和消退（A/E）损伤，并引起腹泻。这种病理形态学特征在肠上皮细胞消失（LEE）的致病岛基因座内编码。LEE容纳3型分泌系统（T3 SS），其在组装时将细菌胞质溶胶桥接到宿主的胞质溶胶，并使细菌能够将数十种效应物运输到宿主中，在宿主中它们劫持调节和信号转导途径并促成细菌定植和疾病。由于LEE在EHEC和EPEC发病机制中的重要性，许多关于这些病原体的研究都集中在其调控上。迄今为止，已经鉴定了超过40种蛋白质因子，其在基因表达的不同层次水平上控制LEE。相比之下，基于RNA的调节机制，收敛于LEE才刚刚开始解开。在这篇小综述中，我们强调了LEE的小RNA（sRNAs）依赖性调控的重大突破，重点是它们的作用机制和/或LEE编码的靶点。
Enterohemorrhagic and enteropathogenic Escherichia coli are gastrointestinal pathogens that disrupt the intestinal microvilli to form attaching and effacing (A/E) lesions on infected cells and cause diarrhea. This pathomorphological trait is encoded within the pathogenicity island locus of enterocyte effacement (LEE). The LEE houses a type 3 secretion system (T3SS), which upon assembly bridges the bacterial cytosol to that of the host and enables the bacterium to traffic dozens of effectors into the host where they hijack regulatory and signal transduction pathways and contribute to bacterial colonization and disease. Owing to the importance of the LEE to EHEC and EPEC pathogenesis, much of the research on these pathogens has centered on its regulation. To date, over 40 proteinaceous factors have been identified that control the LEE at various hierarchical levels of gene expression. In contrast, RNA-based regulatory mechanisms that converge on the LEE have only just begun to be unraveled. In this minireview, we highlight major breakthroughs in small RNAs (sRNAs)-dependent regulation of the LEE, with an emphasis on their mechanisms of action and/or LEE-encoded targets.
登录
查看更多内容
影响因子：
11.3
作者：
Beisel CL;Storz G
通讯作者：
Storz G
影响因子：
3.6
作者：
Durand, Sylvain;Storz, Gisela
通讯作者：
Storz, Gisela
影响因子：
3.2
作者：
Jimenez, Rafael;Cruz-Migoni, Sara B.;Puente, Jose L.
通讯作者：
Puente, Jose L.
影响因子：
64.5
作者：
Kenny, B;DeVinney, R;Finlay, BB
通讯作者：
Finlay, BB
影响因子：
14.9
作者：
Keseler IM;Mackie A;Peralta-Gil M;Santos-Zavaleta A;Gama-Castro S;Bonavides-Martínez C;Fulcher C;Huerta AM;Kothari A;Krummenacker M;Latendresse M;Muñiz-Rascado L;Ong Q;Paley S;Schröder I;Shearer AG;Subhraveti P;Travers M;Weerasinghe D;Weiss V;Collado-Vides J;Gunsalus RP;Paulsen I;Karp PD
通讯作者：
Karp PD