In-depth, high-accuracy proteomics of sea urchin tooth organic matrix.
In-depth, high-accuracy proteomics of sea urchin tooth organic matrix.
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DOI：
10.1186/1477-5956-6-33
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发表时间：
2008-12-09
期刊：
影响因子：
2
通讯作者：
Mann M
中科院分区：
文献类型：
--
作者：
Mann K;Poustka AJ;Mann M
生物矿物中所含的有机基质在调节矿化和决定生物矿物性质方面起着重要作用。然而，生物矿物基质的大部分成分目前尚不清楚。海胆牙是发育生物学和生物矿化的重要模型，但目前发现的基质成分较少。最近发表的purpuratus Strongylocentrotus基因组序列不仅使鉴定潜在编码基质蛋白的基因成为可能，而且通过深入、高精度的蛋白质组学分析，可以直接鉴定骨骼元件基质中含有的蛋白质。在牙粉基质中鉴定出138种蛋白质。这些蛋白质中只有56种以前在测试（壳）和脊柱基质中被鉴定出来。在这些新成分中，有一组有趣的蛋白质，它们含有富含丙氨酸和脯氨酸的中性或碱性基序，由富含酸性甘氨酸的基序隔开。此外，五个蛋白中的四个含有一个或两个预测的Kazal蛋白酶抑制剂结构域。然而，牙基质的主要成分与试验（壳）和脊柱基质中鉴定的一组针状基质蛋白和msp130相关蛋白基本相同。碾碎牙齿与完整牙齿的基质比较显示，已知的晶体内基质蛋白明显稀释，同时一些细胞内蛋白增加。本报告介绍了目前最全面的海胆牙基质蛋白清单。鉴定出的复杂的蛋白质混合物可能反映了矿化过程的许多不同方面。完整的牙齿基质（可能含有成牙细胞残留物）和破碎的牙齿基质之间的比较有助于区分基质成分和细胞残留物的可能贡献。由于基于LC-MS/ ms的方法直接测量肽，我们的结果验证了许多预测的基因，并确认了相应蛋白质的存在。了解该模型系统的组成部分可能会刺激进一步的实验，旨在阐明生物矿物基质成分的结构、功能和相互作用。
The organic matrix contained in biominerals plays an important role in regulating mineralization and in determining biomineral properties. However, most components of biomineral matrices remain unknown at present. In sea urchin tooth, which is an important model for developmental biology and biomineralization, only few matrix components have been identified. The recent publication of the Strongylocentrotus purpuratus genome sequence rendered possible not only the identification of genes potentially coding for matrix proteins, but also the direct identification of proteins contained in matrices of skeletal elements by in-depth, high-accuracy proteomic analysis. We identified 138 proteins in the matrix of tooth powder. Only 56 of these proteins were previously identified in the matrices of test (shell) and spine. Among the novel components was an interesting group of five proteins containing alanine- and proline-rich neutral or basic motifs separated by acidic glycine-rich motifs. In addition, four of the five proteins contained either one or two predicted Kazal protease inhibitor domains. The major components of tooth matrix were however largely identical to the set of spicule matrix proteins and MSP130-related proteins identified in test (shell) and spine matrix. Comparison of the matrices of crushed teeth to intact teeth revealed a marked dilution of known intracrystalline matrix proteins and a concomitant increase in some intracellular proteins. This report presents the most comprehensive list of sea urchin tooth matrix proteins available at present. The complex mixture of proteins identified may reflect many different aspects of the mineralization process. A comparison between intact tooth matrix, presumably containing odontoblast remnants, and crushed tooth matrix served to differentiate between matrix components and possible contributions of cellular remnants. Because LC-MS/MS-based methods directly measures peptides our results validate many predicted genes and confirm the existence of the corresponding proteins. Knowledge of the components of this model system may stimulate further experiments aiming at the elucidation of structure, function, and interaction of biomineral matrix components.
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影响因子：
7
作者：
Ishihama, Y;Oda, Y;Mann, M
通讯作者：
Mann, M
影响因子：
3.2
作者：
Ameye, L;Hermann, R;Dubois, P
通讯作者：
Dubois, P
影响因子：
2.4
作者：
Illies, MR;Peeler, MT;Ettensohn, CA
通讯作者：
Ettensohn, CA
DOI：
10.1073/pnas.0405549101
发表时间：
2004-09-14
影响因子：
11.1
作者：
Olsen, JV;Mann, M
通讯作者：
Mann, M
影响因子：
7
作者：
Olsen, JV;de Godoy, LMF;Mann, M
通讯作者：
Mann, M