A chick neural retina adhesion and survival molecule is a retinol- binding protein
A chick neural retina adhesion and survival molecule is a retinol- binding protein
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鸡神经视网膜粘附和存活分子是视黄醇结合蛋白
作者：
D. Schubert;M. Lacorbiere;F. Esch
一种名为紫癜蛋白的20,000 D蛋白最近从培养的胚胎鸡神经视网膜细胞的生长条件培养基中分离出来（Schubert， D., and M. LaCorbiere, 1985， J. Cell Biol）。， 101:1071- 1077)。紫嘌呤是黏附物的组成成分，通过与细胞表面硫酸肝素蛋白聚糖相互作用促进细胞黏附。它还能延长培养的神经视网膜细胞的存活时间。研究表明，紫癜蛋白是一种分泌性蛋白，其序列与人体肝脏合成的一种转运血液中视黄醇的蛋白——血清视黄醇结合蛋白（RBP）具有同源性。紫癜蛋白与[3H]视黄醇结合，尽管血清蛋白活性不如紫癜蛋白，但紫癜蛋白和鸡血清RBP均能刺激神经视网膜细胞的粘附。然而，Purpurin和血清RBP是不同的分子，因为血清蛋白比Purpurin大约3,000 D，并且具有不同的银染色特征。最后，紫嘌呤支持游离睫状神经节细胞的存活，表明rbp可以作为睫状神经营养因子。
A 20,000-D protein called purpurin has recently been isolated from the growth-conditioned medium of cultured embryonic chick neural retina cells (Schubert, D., and M. LaCorbiere, 1985, J. Cell Biol., 101:1071- 1077). Purpurin is a constituent of adherons and promotes cell-adheron adhesion by interacting with a cell surface heparan sulfate proteoglycan. It also prolongs the survival of cultured neural retina cells. This paper shows that purpurin is a secretory protein that has sequence homology with a human protein synthesized in the liver that transports retinol in the blood, the serum retinol-binding protein (RBP). Purpurin binds [3H]retinol, and both purpurin and chick serum RBP stimulate the adhesion of neural retina cells, although the serum protein is less active than purpurin. Purpurin and the serum RBP are, however, different molecules, for the serum protein is approximately 3,000 D larger than purpurin and has different silver-staining characteristics. Finally, purpurin supports the survival of dissociated ciliary ganglion cells, indicating that RBPs can act as ciliary neurotrophic factors.
使用市售气相测序仪进行多肽微序列分析。
影响因子：
2.9
作者：
Esch,FS
通讯作者：
Esch,FS
高灵敏度氨基酸分析：用少于 100 皮摩尔的肽测定氨基酸组成的方法。
影响因子：
2.9
作者：
Böhlen,P;Schroeder,R
通讯作者：
Schroeder,R
神经营养因子的自动比色微量测定。
影响因子：
2.9
作者：
Manthorpe,M;Fagnani,R;Skaper,SD;Varon,S
通讯作者：
Varon,S