Identification and functional analysis of glutamine transporter in Streptococcus mutans
Identification and functional analysis of glutamine transporter in Streptococcus mutans
复制标题
变形链球菌谷氨酰胺转运蛋白的鉴定及功能分析
DOI：
10.1080/20002297.2020.1797320
复制
发表时间：
2020
影响因子：
4.5
通讯作者：
Matsumoto-Nakano M
中科院分区：
文献类型：
--
作者：
Morikawa Y;Morimoto S;Yoshida E;Naka S;Inaba H;Matsumoto-Nakano M
背景变形链球菌是一种生物膜形成菌，具有多种转运蛋白作为输入/输出分子。其中，PII蛋白家族由调节细菌谷氨酰胺合成的成员组成。目的在本研究中，我们表征了变异链球菌MT 8148中谷氨酰胺转运蛋白的功能。方法SMU.732基因，对应于glnPinS。变异芽孢杆菌（Bacillusmutans）的谷氨酰胺转运蛋白基因与枯草芽孢杆菌（Bacillussubtilis）的谷氨酰胺转运蛋白基因同源。我们构建了aglnP失活的突变株（GEMR）和一个互补株（comp-GEMR），并评估其生物学functions.ResultsGrowth的GEMR是相似的存在和不存在的谷氨酰胺，而MT 8148和comp-GEMR的生长率显着降低谷氨酰胺的存在下相比，其缺乏。此外，由MT 8148和comp-GEMR形成的生物膜显著厚于由GEMR形成的生物膜，而GEMR菌株在酸性环境中的存活率显著低于其他菌株。此外，N-苯基-2-萘胺，用于标记的膜，导致增加的荧光强度的MT 8148和GEMR，虽然这是显着较低，在后者conclusionsThese结果表明，glnPis与谷氨酰胺转运在变形链球菌，特别是进口的谷氨酰胺参与生物膜的形成。
BackgroundStreptococcus mutans, a biofilm-forming bacterium, possesses several transporters that function as import/export molecules. Among them, the PII protein family is composed of members that regulate glutamine synthesis in bacterial species.ObjectiveIn this study, we characterized the function of the glutamine transporter inS. mutansMT8148.MethodsThe SMU.732 gene, corresponding toglnPinS. mutans, is homologous to the glutamine transporter gene inBacillus subtilis. We constructed aglnP-inactivated mutant strain (GEMR) and a complement strain (comp-GEMR) and evaluated their biological functions.ResultsGrowth of GEMR was similar in the presence and absence of glutamine, whereas the growth rates of MT8148 and comp-GEMR were significantly lower in the presence of glutamine as compared to its absence. Furthermore, biofilms formed by MT8148 and comp-GEMR were significantly thicker than that formed by GEMR, while the GEMR strain showed a significantly lower survival rate in an acidic environment than the other strains. Addition of n-phenyl-2-naphthylamine, used to label of the membrane, led to increased fluorescence intensity of MT8148 and GEMR, albeit that was significantly lower in the latter.ConclusionsThese results suggest thatglnPis associated with glutamine transport inS. mutans, especially the import of glutamine involved in biofilm formation.
登录
查看更多内容
影响因子：
2.8
作者：
Lemos, Jose A.;Burne, Robert A.
通讯作者：
Burne, Robert A.
肺炎克雷伯菌 glnB 基因的鉴定：野生型和突变型等位基因的核苷酸序列
DOI：
10.1007/bf00331314
发表时间：
1988
期刊：
Molecular and General Genetics MGG
影响因子：
--
作者：
A. Holtel;M. Merrick
通讯作者：
M. Merrick
影响因子：
--
作者：
DASHPER, SG;RILEY, PF;REYNOLDS, EC
通讯作者：
REYNOLDS, EC
影响因子：
3.1
作者：
Hendriksen, Wouter T.;Kloosterman, Tomas G.;Hermans, Peter W. M.
通讯作者：
Hermans, Peter W. M.
DOI：
10.1073/pnas.95.23.13965
发表时间：
1998-11-10
影响因子：
11.1
作者：
Hsieh, MH;Lam, HM;Coruzzi, G
通讯作者：
Coruzzi, G