Clathrin-mediated endocytosis in budding yeast.

Clathrin-mediated endocytosis in budding yeast.
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DOI:
10.1016/j.tcb.2011.09.001
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发表时间:
2012-01
影响因子:
19
通讯作者:
Drubin, David G.
Drubin, David G.
中科院分区:
生物学1区
文献类型:
--
作者:
Weinberg, Jasper;Drubin, David G.

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Endocytosis in the budding yeast Saccharomyces cerevisiae involves the ordered recruitment, activity and disassembly of nearly 60 proteins at distinct sites on the plasma membrane. Two-color live-cell fluorescence microscopy has proven to be invaluable for in vivo analysis of endocytic proteins: identifying new components, determining the order of protein arrival and dissociation, and revealing even very subtle mutant phenotypes. Yeast genetics and functional genomics facilitate identification of complex interaction networks between endocytic proteins and their regulators. Quantitive datasets produced by these analyses have made theoretical modeling possible. Here, we discuss recent findings on budding yeast endocytosis that have advanced our knowledge of how ~60 endocytic proteins are recruited, regulated by lipid and protein modifications, and disassembled with remarkable regularity.
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