Development and application of a polymicrobial, in vitro, wound biofilm model.
Development and application of a polymicrobial, in vitro, wound biofilm model.
复制标题
DOI：
10.1111/j.1365-2672.2012.05264.x
复制
发表时间：
2012-05
影响因子：
4
通讯作者：
James GA
中科院分区：
文献类型：
--
作者：
Woods J;Boegli L;Kirker KR;Agostinho AM;Durch AM;Delancey Pulcini E;Stewart PS;James GA
本研究的目的是开发一种体外、多微生物、伤口生物膜，能够支持细菌在不同氧气需求下的生长。采用滴流式反应器培养伤口均质液，分离出严格厌氧菌产气荚膜梭菌，并在菌落滴流式反应器模型中采用耐甲氧西林金黄色葡萄球菌（MRSA）、铜绿假单胞菌和产气荚膜梭菌建立三种生物膜模型。平板计数显示MRSA、P. aeruginosa和C. perfringens分别增加到7.39±0.45、10.22±0.22和7.13±0.77 log CFU /膜。采用三种模型评估两种抗菌敷料Curity™AMD和Acticoat™与无菌纱布对照的疗效。对于任何物种，Curity™AMD和纱布上的微生物生长没有显著差异，而Acticoat™被发现显著降低了所有三种物种的生长。利用coloni - dfr，成功地培养了三种生物膜，生物膜显示出独特的结构，由不同的层组成，似乎只由单一物种居住或主要由单一物种居住。本研究的主要成就是在体外模型中分离和生长专性厌氧菌，而无需建立人工厌氧环境。
The goal of this investigation was to develop an in vitro, polymicrobial, wound biofilm capable of supporting the growth of bacteria with variable oxygen requirements. The strict anaerobe Clostridium perfringens was isolated by cultivating wound homogenates using the drip-flow reactor, and a three-species biofilm model was established using methicillin-resistant Staphylococcus aureus (MRSA), Pseudomonas aeruginosa, and C. perfringens in the colony-drip-flow reactor model. Plate counts revealed that MRSA, P. aeruginosa, and C. perfringens grew to 7.39±0.45, 10.22±0.22, and 7.13±0.77 log CFU per membrane, respectively. The three-species model was employed to evaluate the efficacy of two antimicrobial dressings, Curity™ AMD and Acticoat™, compared to sterile gauze controls. Microbial growth on Curity™ AMD and gauze were not significantly different, for any species, whereas Acticoat™ was found to significantly reduce growth for all three species. Using the Colony-DFR, a three-species biofilm was successfully grown, and the biofilms displayed a unique structure consisting of distinct layers that appeared to be inhabited exclusively or predominantly by a single species. The primary accomplishment of this study was the isolation and growth of an obligate anaerobe in an in vitro model without establishing an artificially anaerobic environment.
登录
查看更多内容
影响因子：
3.7
作者：
Dalton T;Dowd SE;Wolcott RD;Sun Y;Watters C;Griswold JA;Rumbaugh KP
通讯作者：
Rumbaugh KP
影响因子：
3.7
作者：
Dowd SE;Wolcott RD;Sun Y;McKeehan T;Smith E;Rhoads D
通讯作者：
Rhoads D
影响因子：
3.1
作者：
Ammons MC;Ward LS;James GA
通讯作者：
James GA
影响因子：
2.3
作者：
Falanga, V
通讯作者：
Falanga, V
影响因子：
3.1
作者：
Leaper, David J
通讯作者：
Leaper, David J