An in vivo polymicrobial biofilm wound infection model to study interspecies interactions.
An in vivo polymicrobial biofilm wound infection model to study interspecies interactions.
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DOI：
10.1371/journal.pone.0027317
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Rumbaugh KP
中科院分区：
文献类型：
--
作者：
Dalton T;Dowd SE;Wolcott RD;Sun Y;Watters C;Griswold JA;Rumbaugh KP
慢性伤口感染通常是多种微生物引起的；然而，大多数体内研究都集中在单一菌种感染上。本项目旨在建立一种体内的、与生物膜相关的多种微生物感染伤口模型，以便研究多种生物膜的动态变化以及与伤口慢性化的关系。由革兰氏阴性菌和革兰氏阳性菌菌株以及需氧菌和厌氧菌组成的多种生物膜在体外培养，然后移植到小鼠的伤口上。这些从体外到体内的多种生物膜移植导致了多种微生物的伤口感染，在整个实验过程中，感染一直保持着四种细菌的异质性。我们观察到，受到多种生物膜感染的受伤小鼠比感染单一菌种的小鼠伤口愈合更差。此外，与单一菌种感染相比，多种微生物伤口感染中的细菌表现出更强的抗菌耐受性。这些数据表明，伤口中不同细菌种类之间的协同作用可能导致愈合延迟和/或抗生素耐受性。
Chronic wound infections are typically polymicrobial; however, most in vivo studies have focused on monospecies infections. This project was designed to develop an in vivo, polymicrobial, biofilm-related, infected wound model in order to study multispecies biofilm dynamics and in relation to wound chronicity. Multispecies biofilms consisting of both Gram negative and Gram positive strains, as well as aerobes and anaerobes, were grown in vitro and then transplanted onto the wounds of mice. These in vitro-to-in vivo multi-species biofilm transplants generated polymicrobial wound infections, which remained heterogeneous with four bacterial species throughout the experiment. We observed that wounded mice given multispecies biofilm infections displayed a wound healing impairment over mice infected with a single-species of bacteria. In addition, the bacteria in the polymicrobial wound infections displayed increased antimicrobial tolerance in comparison to those in single species infections. These data suggest that synergistic interactions between different bacterial species in wounds may contribute to healing delays and/or antibiotic tolerance.
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影响因子：
3.7
作者：
Dowd SE;Wolcott RD;Sun Y;McKeehan T;Smith E;Rhoads D
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