RNA sequencing provides insights into the evolution of lettuce and the regulation of flavonoid biosynthesis.
RNA sequencing provides insights into the evolution of lettuce and the regulation of flavonoid biosynthesis.
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RNA测序为了解生菜的进化和类黄酮生物合成的调控提供了见解
DOI：
10.1038/s41467-017-02445-9
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发表时间：
2017-12-22
影响因子：
16.6
通讯作者：
Kuang H
中科院分区：
文献类型：
--
作者：
Zhang L;Su W;Tao R;Zhang W;Chen J;Wu P;Yan C;Jia Y;Larkin RM;Lavelle D;Truco MJ;Chin-Wo SR;Michelmore RW;Kuang H
不同园艺类型的莴苣表现出巨大的形态差异。然而，不同园艺类型莴苣的驯化和分化的分子基础仍不清楚。在这里，我们报告了从主要园艺类型和野生近缘种中取样的240个生菜材料的RNA测序，产生110万个单核苷酸多态性（snp）。人口统计学模型表明，莴苣的驯化事件只有一次。我们确定了一系列区域作为假定的选择性扫描，分别发生在驯化和分化期间。全基因组关联研究（GWAS）鉴定出5311个表达数量性状位点（eQTL），调控4105个基因的表达，其中9个eQTL调控与类黄酮生物合成相关的基因。叶片颜色的GWAS检测到莴苣叶片花青素变异的六个候选基因座。本研究为生菜花色苷的驯化和积累提供了全面的认识，为培育营养价值更高的品种提供了依据。
Different horticultural types of lettuce exhibit tremendous morphological variation. However, the molecular basis for domestication and divergence among the different horticultural types of lettuce remains unknown. Here, we report the RNA sequencing of 240 lettuce accessions sampled from the major horticultural types and wild relatives, generating 1.1 million single-nucleotide polymorphisms (SNPs). Demographic modeling indicates that there was a single domestication event for lettuce. We identify a list of regions as putative selective sweeps that occurred during domestication and divergence, respectively. Genome-wide association studies (GWAS) identify 5311 expression quantitative trait loci (eQTL) regulating the expression of 4105 genes, including nine eQTLs regulating genes associated with flavonoid biosynthesis. GWAS for leaf color detects six candidate loci responsible for the variation of anthocyanins in lettuce leaves. Our study provides a comprehensive understanding of the domestication and the accumulation of anthocyanins in lettuce, and will facilitate the breeding of cultivars with improved nutritional value.
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影响因子：
4.5
作者：
Gutenkunst RN;Hernandez RD;Williamson SH;Bustamante CD
通讯作者：
Bustamante CD
影响因子：
3.3
作者：
Breseghello, F;Sorrells, ME
通讯作者：
Sorrells, ME
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7
作者：
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通讯作者：
Reich, David
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1.2
作者：
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通讯作者：
Ruden, Douglas M.
影响因子：
16.6
作者：
Fu, Junjie;Cheng, Yanbing;Wang, Guoying
通讯作者：
Wang, Guoying