Chloroplast elongation factor BcEF-Tu responds to turnip mosaic virus infection and heat stress in non-heading Chinese cabbage

Chloroplast elongation factor BcEF-Tu responds to turnip mosaic virus infection and heat stress in non-heading Chinese cabbage
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叶绿体延伸因子 BcEF-Tu 对不结球白菜萝卜花叶病毒感染和热应激的响应

DOI:
10.1007/s10535-014-0419-4
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发表时间:
2014-05
期刊:
影响因子:
1.5
通讯作者:
Hou, X. -L.
Hou, X. -L.
中科院分区:
生物学4区
文献类型:
--
作者:
Li, Y. -X.;Zhang, C. -W.;Li, Y.;Hou, X. -L.

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真核细胞延伸因子Tu与热应激和病毒感染的反应有关。本研究利用芜菁花叶病毒(TuMV)感染不结球白菜叶片的cDNA文库,对芜菁花叶病毒应答基因BcLRK 01进行了研究。通过酵母双杂交系统获得了叶绿体延伸因子Tu的编码基因BcEF-Tu,并验证了其与BcLRK 01基因的相互作用。TuMV感染抑制该基因的表达,而热应激诱导其表达。过表达BcEF-Tu可提高大肠杆菌转化子在热应激条件下的存活率。这些结果表明,延伸因子BcEF-Tu响应TuMV感染和热应激。这是不结球白菜叶绿体EF-Tu的首次报道,为EF-Tu的功能研究提供了理论依据。
Eukaryotic elongation factor Tu has been implicated in responses to heat stress and viral infection. In this study, the turnip mosaic virus (TuMV)-response geneBcLRK01, which encodes a leucine-rich repeat receptor-like kinase, was probed using the cDNA library of TuMV-infected leaves of non-heading Chinese cabbage (Brassica campestrisssp.chinensis). TheBcEF-Tugene, which encodes chloroplast elongation factor Tu, was obtained and verified by a yeast two-hybrid system to interact with theBcLRK01gene. TuMV infection depressed the expression of this gene, whereas a heat stress induced its expression. Overexpression ofBcEF-Tuenhanced the viability ofEscherichia colitransformants under the heat stress. These results demonstrate that elongation factor BcEF-Tu responded to the TuMV infection and heat stress. This is the first report on chloroplast EF-Tu in non-heading Chinese cabbage which provides a theoretical basis for the functional research of EF-Tu.
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