Enhancement of recombinant adenovirus vaccine-induced primary but not secondary systemic and mucosal immune responses by all-trans retinoic acid.-猫眼课题宝

全反式视黄酸增强重组腺病毒疫苗诱导的初级而非次级全身和粘膜免疫反应。

DOI：

10.1016/j.vaccine.2014.04.028

发表时间：

2014

期刊：

Vaccine

影响因子：

5.5

通讯作者：

中科院分区：

医学3区

文献类型：

作者：

Tuyishime,Steven;Haut,LarissaH;Zhu,Caihong;Ertl,HildegundCJ

疫苗接种是增强针对粘膜病原体的免疫应答的重要工具。肌肉注射腺病毒载体已被证明是系统和粘膜免疫应答的强诱导剂。Ad疫苗接种后免疫应答的进一步增强是非常期望的。全反式维甲酸（ATRA）是一种具有生物活性的维生素A代谢产物，已被探索作为疫苗接种后原发性免疫应答的佐剂。在这项研究中，我们研究了ATRA对异源Ad prime加强方案的影响。ATRA联合给药在引发增加粘膜和全身抗体反应，以及粘膜，但不是全身的CD8 + T细胞反应。然而，无论ATRA是在引发时、在加强时还是在两次免疫时施用，这种效果在加强后都不再明显。我们的研究结果证实，全反式维甲酸作为一种佐剂的初级免疫反应，并建议，佐剂的效果不延伸到二次免疫反应。

Vaccination is an important tool for enhancing immune responses against mucosal pathogens. Intramuscularly administered adenovirus (Ad) vectors have been demonstrated to be strong inducers of both systemic and mucosal immune responses. Further enhancement of immune responses following Ad vaccination is highly desirable. All-transretinoic acid (ATRA), a biologically active vitamin A metabolite, has been explored as an adjuvant for primary immune responses following vaccination. In this study, we investigated the effect of ATRA on a heterologous Ad prime boost regimen. ATRA co-administration during priming increased mucosal and systemic antibody responses as well as mucosal but not systemic CD8+T cell responses. However, this effect was no longer apparent after boosting regardless of whether ATRA was administered at the time of priming, at the time of boosting, or at both immunizations. Our findings confirm ATRA as an adjuvant for primary immune responses and suggest that the adjuvant effect does not extend to secondary immune responses.