Comprehensive study of gene and microRNA expression related to epithelial-mesenchymal transition in prostate cancer.
Comprehensive study of gene and microRNA expression related to epithelial-mesenchymal transition in prostate cancer.
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DOI：
10.1371/journal.pone.0113700
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Leite KR
中科院分区：
文献类型：
--
作者：
Katz B;Reis ST;Viana NI;Morais DR;Moura CM;Dip N;Silva IA;Iscaife A;Srougi M;Leite KR
前列腺癌是男性中最常见的癌症，大多数患者在诊断时都是局部疾病。然而，4%已经出现转移性疾病。上皮-间质转化是癌发生的一个基本过程，已被证明与前列腺癌的进展有关。上皮-间质转化的主要事件是转录因子对e -钙粘蛋白的抑制，但这一过程也受到microrna的调节。本研究的目的是分析局部前列腺癌和转移性前列腺癌细胞系中参与上皮-间质转化的基因和microRNA表达及其与临床病理结果的相关性。我们研究了51例经根治性前列腺切除术治疗的局限性前列腺癌（PCa）患者和三种转移性前列腺癌细胞系（LNCaP, DU145, PC3）的新鲜冷冻组织样本。实时荧光定量PCR检测10个基因和18个mirna的表达情况。根据Gleason评分、病理分期、术前PSA、生化复发、危险组与临床病理表现的相关性进行分组。大多数局部PCa病例表现为上皮表型，E-cadherin过表达，间充质标志物过表达。MiRNA-200家族成员和miRNAs 203、205、183、373和21过表达，miRNAs 9、495、29b和1过表达。miRNAs 200b、30a和1的低表达水平与病理分期有显著相关性。miR-200b的低表达也与Gleason评分≥8和更短的生化无复发生存期相关。高危组miR-30a低表达，Vimentin和Twist1高表达。与原发肿瘤相比，转移细胞系miR-183和Twist1的表达水平明显升高。综上所述，miRNAs 200b、30a、1和183以及Twist1和Vimentin基因可能在前列腺癌的进展中发挥重要作用，并最终可能成为重要的预后标志物。
Prostate cancer is the most common cancer in men, and most patients have localized disease at the time of diagnosis. However, 4% already present with metastatic disease. Epithelial-mesenchymal transition is a fundamental process in carcinogenesis that has been shown to be involved in prostate cancer progression. The main event in epithelial-mesenchymal transition is the repression of E-cadherin by transcription factors, but the process is also regulated by microRNAs. The aim of this study was to analyze gene and microRNA expression involved in epithelial-mesenchymal transition in localized prostate cancer and metastatic prostate cancer cell lines and correlate with clinicopathological findings. We studied 51 fresh frozen tissue samples from patients with localized prostate cancer (PCa) treated by radical prostatectomy and three metastatic prostate cancer cell lines (LNCaP, DU145, PC3). The expression of 10 genes and 18 miRNAs were assessed by real-time PCR. The patients were divided into groups according to Gleason score, pathological stage, preoperative PSA, biochemical recurrence, and risk group for correlation with clinicopathological findings. The majority of localized PCa cases showed an epithelial phenotype, with overexpression of E-cadherin and underexpression of the mesenchymal markers. MiRNA-200 family members and miRNAs 203, 205, 183, 373, and 21 were overexpressed, while miRNAs 9, 495, 29b, and 1 were underexpressed. Low-expression levels of miRNAs 200b, 30a, and 1 were significantly associated with pathological stage. Lower expression of miR-200b was also associated with a Gleason score ≥8 and shorter biochemical recurrence-free survival. Furthermore, low-expression levels of miR-30a and high-expression levels of Vimentin and Twist1 were observed in the high-risk group. Compared with the primary tumor, the metastatic cell lines showed significantly higher expression levels of miR-183 and Twist1. In summary, miRNAs 200b, 30a, 1, and 183 and the genes Twist1 and Vimentin might play important roles in the progression of prostate cancer and may eventually become important prognostic markers.
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影响因子：
8.8
作者：
Kojima, S.;Chiyomaru, T.;Kawakami, K.;Yoshino, H.;Enokida, H.;Nohata, N.;Fuse, M.;Ichikawa, T.;Naya, Y.;Nakagawa, M.;Seki, N.
通讯作者：
Seki, N.
影响因子：
5.2
作者：
Kong, Dejuan;Li, Yiwei;Wang, Zhiwei;Banerjee, Sanjeev;Ahmad, Aamir;Kim, Hyeong-Reh Choi;Sarkar, Fazlul H.
通讯作者：
Sarkar, Fazlul H.
影响因子：
21.3
作者：
Batlle, E;Sancho, E;de Herreros, AG
通讯作者：
de Herreros, AG
影响因子：
11.2
作者：
Bracken, Cameron P.;Gregory, Philip A.;Goodall, Gregory J.
通讯作者：
Goodall, Gregory J.
影响因子：
14.9
作者：
Hudson RS;Yi M;Esposito D;Watkins SK;Hurwitz AA;Yfantis HG;Lee DH;Borin JF;Naslund MJ;Alexander RB;Dorsey TH;Stephens RM;Croce CM;Ambs S
通讯作者：
Ambs S