First comprehensive analysis of lysine succinylation in paper mulberry (Broussonetia papyrifera).
First comprehensive analysis of lysine succinylation in paper mulberry (Broussonetia papyrifera).
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DOI：
10.1186/s12864-021-07567-5
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发表时间：
2021-04-10
期刊：
影响因子：
4.4
通讯作者：
Chen C
中科院分区：
文献类型：
--
作者：
Dong Y;Li P;Li P;Chen C
赖氨酸琥珀酰化是一种天然存在的翻译后修饰（PTM），在生物体中普遍存在。赖氨酸琥珀酰化在调节蛋白质结构和功能以及细胞代谢中起着重要作用。全球赖氨酸琥珀酰化在蛋白质组水平已被确定在各种物种，然而，有限的信息赖氨酸琥珀酰化在植物物种，特别是纸桑葚，是可用的。桑葚不仅是一种重要的中药植物，也是一种具有重要经济价值的树种。桑葚分布于中国的温带和热带地区。研究了赖氨酸琥珀酰化修饰对桑葚生长发育及生理的影响。共2097个赖氨酸琥珀酰化位点被确定在935蛋白与柠檬酸循环（TCA循环），乙醛酸和二羧酸代谢，核糖体和氧化磷酸化，这些途径在光合生物的碳固定中发挥作用，并可能受到调节赖氨酸琥珀酰化。修饰后的蛋白质分布在多个亚细胞区室中，并参与各种各样的生物过程，如光合作用和卡尔文-本森循环。赖氨酸琥珀酰化蛋白可能在代谢中起关键的调节作用，主要是在光合作用和氧化磷酸化，以及在许多其他细胞过程中。除了大量与光合作用和氧化磷酸化相关的琥珀酰化蛋白质外，一些与TCA循环相关的蛋白质也被琥珀酰化。本研究可为进一步研究琥珀酰化对桑葚生理生化的下游影响提供参考。在线版本包含补充材料，可通过10.1186/s12864-021-07567-5获得。
Lysine succinylation is a naturally occurring post-translational modification (PTM) that is ubiquitous in organisms. Lysine succinylation plays important roles in regulating protein structure and function as well as cellular metabolism. Global lysine succinylation at the proteomic level has been identified in a variety of species; however, limited information on lysine succinylation in plant species, especially paper mulberry, is available. Paper mulberry is not only an important plant in traditional Chinese medicine, but it is also a tree species with significant economic value. Paper mulberry is found in the temperate and tropical zones of China. The present study analyzed the effects of lysine succinylation on the growth, development, and physiology of paper mulberry. A total of 2097 lysine succinylation sites were identified in 935 proteins associated with the citric acid cycle (TCA cycle), glyoxylic acid and dicarboxylic acid metabolism, ribosomes and oxidative phosphorylation; these pathways play a role in carbon fixation in photosynthetic organisms and may be regulated by lysine succinylation. The modified proteins were distributed in multiple subcellular compartments and were involved in a wide variety of biological processes, such as photosynthesis and the Calvin-Benson cycle. Lysine-succinylated proteins may play key regulatory roles in metabolism, primarily in photosynthesis and oxidative phosphorylation, as well as in many other cellular processes. In addition to the large number of succinylated proteins associated with photosynthesis and oxidative phosphorylation, some proteins associated with the TCA cycle are succinylated. Our study can serve as a reference for further proteomics studies of the downstream effects of succinylation on the physiology and biochemistry of paper mulberry. The online version contains supplementary material available at 10.1186/s12864-021-07567-5.
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