Methods to assess Drosophila heart development, function and aging.

Methods to assess Drosophila heart development, function and aging.
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DOI:
10.1016/j.ymeth.2014.03.031
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发表时间:
2014-06-15
期刊:
影响因子:
4.8
通讯作者:
Bodmer, Rolf
Bodmer, Rolf
中科院分区:
生物学3区
文献类型:
--
作者:
Ocorr, Karen;Vogler, Georg;Bodmer, Rolf

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近年来,果蝇的心脏已经成为人类心脏病许多不同方面的既定模型。这一模型可以确定先天性心脏病和心肌病的致病机制,并可以研究遗传、代谢和年龄对心脏功能的影响。在这篇综述中,我们讨论了目前用于分析果蝇心脏结构和功能的方法,如用于推断心脏功能和表现的光学方法,用于表征心脏组织特性的电生理和力学方法,并总结了用于心脏发育和成体结构研究的组织学技术。
In recent years the Drosophila heart has become an established model of many different aspects of human cardiac disease. This model has allowed identification of disease-causing mechanisms underlying congenital heart disease and cardiomyopathies and has permitted the study underlying genetic, metabolic and age-related contributions to heart function. In this review we discuss methods currently employed in the analysis of the Drosophila heart structure and function, such as optical methods to infer heart function and performance, electrophysiological and mechanical approaches to characterize cardiac tissue properties, and conclude with histological techniques used in the study of heart development and adult structure.
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