Biomimicry Promotes the Efficiency of a 10-Step Sequential Enzymatic Reaction on Nanoparticles, Converting Glucose to Lactate.
Biomimicry Promotes the Efficiency of a 10-Step Sequential Enzymatic Reaction on Nanoparticles, Converting Glucose to Lactate.
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DOI：
10.1002/anie.201609495
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发表时间：
2017-01-02
影响因子：
16.6
通讯作者：
Travis, Alexander J.
中科院分区：
文献类型：
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作者：
Mukai, Chinatsu;Gao, Lizeng;Nelson, Jacquelyn L.;Lata, James P.;Cohen, Roy;Wu, Lauren;Hinchman, Meleana M.;Bergkvist, Magnus;Sherwood, Robert W.;Zhang, Sheng;Travis, Alexander J.
为了使纳米生物技术发挥其潜力，复杂的有机-无机系统必须不断发展，以利用多种生物成分的顺序功能。关键的挑战存在：固定化酶可以阻断底物结合位点或禁止构象变化，底物组成可以干扰活性，多步骤反应有中间体扩散的风险。因此，报道的最复杂的拴链反应只涉及3种酶。受定向固定糖酵解酶在哺乳动物精子纤维鞘上的启发，我们展示了一个由10种酶拴在纳米颗粒上的复杂反应。虽然单个酶的效率在溶液中更高，但通过葡萄糖转化为乳酸的10步途径的效率在拴绳时显着更高。据我们所知，这是所描述的最复杂的有机-无机系统，它表明，在混合系统中，拴系的、多步骤的生物途径可以被重组，以执行诸如能量生产或分子货物输送等功能。
For nanobiotechnology to achieve its potential, complex organic-inorganic systems must grow to utilize the sequential functions of multiple biological components. Critical challenges exist: immobilizing enzymes can block substrate-binding sites or prohibit conformational changes, substrate composition can interfere with activity, and multistep reactions risk diffusion of intermediates. As a result, the most complex tethered reaction reported involves only 3 enzymes. Inspired by the oriented immobilization of glycolytic enzymes on the fibrous sheath of mammalian sperm, here we show a complex reaction of 10 enzymes tethered to nanoparticles. Although individual enzyme efficiency was higher in solution, the efficacy of the 10-step pathway measured by conversion of glucose to lactate was significantly higher when tethered. To our knowledge, this is the most complex organic-inorganic system described, and it shows that tethered, multi-step biological pathways can be reconstituted in hybrid systems to carry out functions such as energy production or delivery of molecular cargo.
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影响因子：
14.8
作者：
Bujara, Matthias;Schuemperli, Michael;Panke, Sven
通讯作者：
Panke, Sven
影响因子：
4.1
作者：
WELCH, P;SCOPES, RK
通讯作者：
SCOPES, RK
DOI：
10.1002/anie.199200561
发表时间：
1992-01-01
期刊：
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION IN ENGLISH
影响因子：
--
作者：
FESSNER, WD;EYRISCH, O
通讯作者：
EYRISCH, O
影响因子：
38.3
作者：
通讯作者：
--
影响因子：
4.7
作者：
You, Chun;Zhang, Y. -H. Percival
通讯作者：
Zhang, Y. -H. Percival