MMP induced by Gr-1+ cells are crucial for recruitment of Th cells into the airways.
MMP induced by Gr-1+ cells are crucial for recruitment of Th cells into the airways.
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DOI：
10.1002/eji.200838985
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发表时间：
2009-08
影响因子：
5.4
通讯作者：
Chaplin, David D.
中科院分区：
文献类型：
--
作者：
Jung, Yong Woo;Zindl, Carlene L.;Lai, Jen-Feng;Weaver, Casey T.;Chaplin, David D.
Th 2淋巴细胞传递诱导哮喘气道炎症的重要信号。我们以前发现，气道抗原激发诱导Gr-1+中性粒细胞的募集之前，募集的Th 2细胞。因此，我们研究了Gr-1+细胞是否有助于Th 2依赖性气道炎症的发展。使用RB 6 - 8 C5单克隆抗体全身性耗竭Gr-1+细胞减少鼻内抗原激发后的Th 2细胞募集。鼻内抗原攻击后12 h，小鼠肺组织中基质金属蛋白酶（MMP）-9和基质金属蛋白酶组织抑制剂-1（TIMP-1）mRNA水平均上调。TIMP-1的上调不依赖于Gr-1+细胞，而MMP-9 RNA的上调和总明胶分解活性在Gr-1+细胞耗竭的小鼠中显著降低。在24小时后的挑战，总肺胶原溶解活性也上调，在Gr-1+细胞依赖性的方式。MMP-8和MMP-9的全身抑制减少了Th细胞的气道募集，导致嗜酸性粒细胞炎症显著减少。这些数据表明，通过气道的抗原激发激活Gr-1+细胞，并因此MMPs，以促进气道炎症反应中Th细胞的募集。
Th2 lymphocytes deliver essential signals for induction of asthmatic airway inflammation. We previously found that airway antigen challenge induces recruitment of Gr-1+ neutrophils prior to the recruitment of Th2 cells. We examined, therefore, whether Gr-1+ cells contribute to the development of Th2-dependent airway inflammation. Systemic depletion of Gr-1+ cells using the RB6-8C5 monoclonal antibody reduced Th2 cell recruitment following intranasal antigen challenge. The levels of both matrix metalloproteinase (MMP)-9 and the tissue inhibitor of matrix metalloproteinases-1 (TIMP-1) mRNA were up-regulated in the lungs of mice 12 h after intranasal antigen challenge. Up-regulation of TIMP-1 was independent of Gr-1+ cells, whereas up-regulation of MMP-9 RNA and total gelatinolytic activity were dramatically reduced in mice depleted of Gr-1+ cells. At 24 h after challenge, total lung collagenolytic activity was also up-regulated, in a Gr-1+ cell-dependent fashion. Systemic inhibition of MMP-8 and MMP-9 reduced the airway recruitment of Th cells, resulting in significantly reduced eosinophilic inflammation. These data suggest that antigen challenge via the airway activates Gr-1+ cells and consequently MMPs to facilitate the recruitment of Th cells in the airway inflammatory response.
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DOI：
10.1097/00004647-199906000-00005
发表时间：
1999-06-01
影响因子：
6.3
作者：
Heo, JH;Lucero, J;del Zoppo, GJ
通讯作者：
del Zoppo, GJ
影响因子：
2.8
作者：
Nocker, RET;Out, TA;van der Zee, JS
通讯作者：
van der Zee, JS
影响因子：
4.8
作者：
Hermant, B;Bibert, S;Gulino-Debrac, D
通讯作者：
Gulino-Debrac, D
影响因子：
9.6
作者：
Gibson, PG;Simpson, JL;Saltos, N
通讯作者：
Saltos, N
DOI：
10.1084/jem.20040035
发表时间：
2004-07-05
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
de Heer HJ;Hammad H;Soullié T;Hijdra D;Vos N;Willart MA;Hoogsteden HC;Lambrecht BN
通讯作者：
Lambrecht BN