Metagenomic insights into S(0) precipitation in a terrestrial subsurface lithoautotrophic ecosystem.
Metagenomic insights into S(0) precipitation in a terrestrial subsurface lithoautotrophic ecosystem.
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DOI：
10.3389/fmicb.2014.00756
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发表时间：
2014
影响因子：
5.2
通讯作者：
Macalady JL
中科院分区：
文献类型：
--
作者：
Hamilton TL;Jones DS;Schaperdoth I;Macalady JL
在意大利的Frasassi和阿夸桑塔泰尔梅洞穴系统主机孤立的岩石自养生态系统的特点是硫氧化生物膜高达50%的S（0）的质量。生物膜中微生物类群对S（0）生产和消耗的净贡献知之甚少，这对理解地质硫矿床的形成以及硫氧化自养生物的生态位具有影响。丝状细菌ε-变形菌是Frasassi和阿夸桑塔泰尔梅溪流中主要的生物膜建筑师，相对于其他主要的生物膜形成种群，它们在高硫化物、低氧环境中定居。8个生物膜样品的宏基因组测序表明存在多样性和丰富的Epsilonproteobacteria。无论生物膜形态、温度或水化学的差异如何，类Sulfurovum生物的种群是最丰富的Epsilonproteobacteria。在组装和合并宏基因组数据后，我们检索了四个几乎完整的类硫菌生物体基因组以及一个硫曲菌属。分组和组装的宏基因组数据的分析表明，ε变形菌是自养的，因此提供有机碳的孤立的地下生态系统。硫化物-醌氧化还原酶（Sqr）的多个同系物，以及不完全或不存在的Sox途径，表明洞穴类Sulfurovum-like Epsilonproteobacteria使用O2或硝酸盐作为末端电子受体将硫化物不完全氧化为S（0），这与先前的证据一致，即它们在高溶解硫化物与氧比率的生态位中最成功。相比之下，我们回收的同源物的完整补体的Sox蛋白隶属于γ-变形菌，并与丰富的Sulfuricurvum属。和弓形杆菌属，这表明这些种群能够将硫化物完全氧化为硫酸盐。这些和其他基因组数据提供了新的线索，生理和遗传潜力的大部分未开垦和生态成功的洞穴Sulfurovum样人口，并建议他们在地下S（0）的形成中发挥不可或缺的作用。
The Frasassi and Acquasanta Terme cave systems in Italy host isolated lithoautotrophic ecosystems characterized by sulfur-oxidizing biofilms with up to 50% S(0) by mass. The net contributions of microbial taxa in the biofilms to production and consumption of S(0) are poorly understood and have implications for understanding the formation of geological sulfur deposits as well as the ecological niches of sulfur-oxidizing autotrophs. Filamentous Epsilonproteobacteria are among the principal biofilm architects in Frasassi and Acquasanta Terme streams, colonizing high-sulfide, low-oxygen niches relative to other major biofilm-forming populations. Metagenomic sequencing of eight biofilm samples indicated the presence of diverse and abundant Epsilonproteobacteria. Populations of Sulfurovum-like organisms were the most abundant Epsilonproteobacteria regardless of differences in biofilm morphology, temperature, or water chemistry. After assembling and binning the metagenomic data, we retrieved four nearly-complete genomes of Sulfurovum-like organisms as well as a Sulfuricurvum spp. Analyses of the binned and assembled metagenomic data indicate that the Epsilonproteobacteria are autotrophic and therefore provide organic carbon to the isolated subsurface ecosystem. Multiple homologs of sulfide-quinone oxidoreductase (Sqr), together with incomplete or absent Sox pathways, suggest that cave Sulfurovum-like Epsilonproteobacteria oxidize sulfide incompletely to S(0) using either O2 or nitrate as a terminal electron acceptor, consistent with previous evidence that they are most successful in niches with high dissolved sulfide to oxygen ratios. In contrast, we recovered homologs of the complete complement of Sox proteins affiliated Gammaproteobacteria and with less abundant Sulfuricurvum spp. and Arcobacter spp., suggesting that these populations are capable of the complete oxidation of sulfide to sulfate. These and other genomic data presented here offer new clues into the physiology and genetic potential of the largely uncultivated and ecologically successful cave Sulfurovum-like populations, and suggest that they play an integral role in subsurface S(0) formation.
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影响因子：
4.3
作者：
Borisov, Vitaliy B.;Gennis, Robert B.;Hemp, James;Verkhovsky, Michael I.
通讯作者：
Verkhovsky, Michael I.
影响因子：
4.4
作者：
Dalsgaard, T;Thamdrup, B
通讯作者：
Thamdrup, B
影响因子：
5.2
作者：
Gregersen LH;Bryant DA;Frigaard NU
通讯作者：
Frigaard NU
影响因子：
6.5
作者：
Anisimova, Maria;Gascuel, Olivier
通讯作者：
Gascuel, Olivier
影响因子：
2.9
作者：
Canfield, DE
通讯作者：
Canfield, DE