Neural stem cells promote glioblastoma formation in nude mice
Neural stem cells promote glioblastoma formation in nude mice
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神经干细胞促进裸鼠胶质母细胞瘤形成
DOI：
10.1007/s12094-019-02087-x
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发表时间：
2019-04
影响因子：
3.4
通讯作者：
X.Yu
中科院分区：
文献类型：
--
作者：
J.Wang;J.Liu;H.Meng;Y.Guan;Y.Yin;Z.Zhao;G.Sun;A.Wu;L.Chen;X.Yu
目的神经干细胞（Neural stem cells，NSCs）具有自我更新和神经发生的能力，近年来，大量研究表明NSCs在神经损伤、缺血性卒中、脑炎症和神经退行性疾病中的作用。我们集中在神经干细胞与胶质母细胞瘤的关系，因为我们已经发现，复发性胶质母细胞瘤倾向于来自脑室下区（SVZ），神经干细胞居住。我们想弄清楚神经干细胞是否参与胶质母细胞瘤的复发。细胞计数试剂盒-8用于测量细胞的增殖。通过伤口愈合和transwell实验检测迁移能力，并在裸鼠中证实肿瘤形成能力。结果我们在实验中发现，神经干细胞促进胶质母细胞瘤细胞系Ln 229的增殖，Ln 229细胞的迁移能力是由与神经干细胞共培养激发的。当与NSC共移植时，Ln 229细胞在裸鼠中的肿瘤形成也被加速。结论NSCs在胶质母细胞瘤复发过程中可能参与了肿瘤的形成，为胶质母细胞瘤的治疗提供了依据。通过对瘤旁SVZ组织的预处理，可能有助于尽可能延缓胶质母细胞瘤的复发。
PurposeNeural stem cells (NSCs) have been characterized with the ability of self-renewal and neurogenesis, which has inspired lots of studies to clarify the functions of NSCs in neural injury, ischemic stroke, brain inflammation and neurodegenerative diseases. We focused on the relationship of NSCs with glioblastoma, since we have discovered that recurrent glioblastomas were inclined to be derived from subventricular zone (SVZ), where NSCs reside. We want to clarify whether NSCs are involved in glioblastoma relapse.MethodsImmunocytochemistry was used to confirm the stemness of NSCs. The Cell Counting Kit-8 was used to measure the proliferation of cells. Migration abilities were examined by wound healing and transwell assays, and tumor formation abilities were confirmed in nude mice.ResultsWe found in experiments that NSCs promoted proliferation of a glioblastoma cell line-Ln229, the migration ability of Ln229 cells was motivated by co-cultured with NSCs. Tumor formation of Ln229 cells was also accelerated in nude mice when co-transplanted with NSCs. In immunohistochemistry, we found that the Sox2- and Ki67-positive cells were much higher in co-transplanted groups than that of control groups.ConclusionsThese results imply the potential role that NSCs play in speeding up tumor formation in the process of glioblastoma relapse, providing the basis for dealing with newly diagnosed glioblastoma patients, which may help postpone the recurrence of glioblastoma as far as possible through preprocessing the tumor-adjacent SVZ tissue.
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影响因子：
64.5
作者：
Kim J;Woo AJ;Chu J;Snow JW;Fujiwara Y;Kim CG;Cantor AB;Orkin SH
通讯作者：
Orkin SH
影响因子：
158.5
作者：
Stupp, R;Mason, WP;Ryan, G
通讯作者：
Ryan, G
影响因子：
10.6
作者：
Keon Mahmoudi;A. Ezrin;C. Hadjipanayis
通讯作者：
Keon Mahmoudi;A. Ezrin;C. Hadjipanayis
影响因子：
3.5
作者：
Ellingson, B. M.;Lai, A.;Cloughesy, T. F.
通讯作者：
Cloughesy, T. F.
影响因子：
11.2
作者：
Galli, R;Binda, E;Vescovi, A
通讯作者：
Vescovi, A