Sperm entry into the egg cell induces the progression of karyogamy in rice zygotes.
Sperm entry into the egg cell induces the progression of karyogamy in rice zygotes.
复制标题
精子进入卵细胞诱导水稻受精卵核配的进展。
DOI：
10.1093/pcp/pcz077
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发表时间：
2019
影响因子：
4.9
通讯作者：
Okamoto T.
中科院分区：
文献类型：
--
作者：
Ohnishi Y*;Kokubu I;Kinoshita T;Okamoto T.
细胞核增生是植物胚胎发生的先决条件，细胞核结构的动态变化和基因表达模式的建立是细胞核发育的必要条件。然而，雄性和雌性配子在细胞核增生过程中的确切作用仍然是难以捉摸的。在这里，我们展示了精子细胞具有驱动稳定和快速核聚变的独特特性。当我们在体外使卵细胞与精子细胞受精时，受精卵中的雄性和雌性细胞核立即融合。当两个卵细胞被人工融合时，这种快速的核聚变不会发生。然而，通过额外的精子进入或外源钙的应用可以加速两个卵细胞核的融合，这表明通过精子进入卵细胞有效地增加细胞质内Ca2+水平可能有助于细胞核增生。与受精卵相比，卵-卵融合细胞在早期发育阶段后无法增殖。我们的转录分析也揭示了合子基因在合子中的快速激活，而在没有雄性贡献的融合细胞中没有表达。因此，男性精子细胞有能力立即引起核细胞增生，并在受精卵中建立适当的基因表达模式。
Karyogamy is a prerequisite event for plant embryogenesis, in which dynamic changes in nuclear architecture and the establishment of appropriate gene expression patterns must occur. However, the precise role of the male and female gametes in the progression of karyogamy still remains elusive. Here, we show that the sperm cell possesses the unique property to drive steady and swift nuclear fusion. When we fertilized egg cells with sperm cells in vitro, the immediate fusion of the male and female nuclei in the zygote progressed. This rapid nuclear fusion did not occur when two egg cells were artificially fused. However, the nuclear fusion of two egg nuclei could be accelerated by additional sperm entry or the exogenous application of calcium, suggesting that possible increase of cytosolic Ca2+level via sperm entry into the egg cell efficiently can facilitate karyogamy. In contrast to zygotes, the egg–egg fusion cells failed to proliferate beyond an early developmental stage. Our transcriptional analyses also revealed the rapid activation of zygotic genes in zygotes, whereas there was no expression in fused cells without the male contribution. Thus, the male sperm cell has the ability to cause immediate karyogamy and to establish appropriate gene expression patterns in the zygote.
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影响因子：
4.9
作者：
Okamoto, T;Higuchi, K;Kranz, E
通讯作者：
Kranz, E
影响因子：
2.8
作者：
Yukinosuke Ohnishi;T. Okamoto
通讯作者：
Yukinosuke Ohnishi;T. Okamoto
影响因子：
56.9
作者：
Sprunck, Stefanie;Rademacher, Svenja;Dresselhaus, Thomas
通讯作者：
Dresselhaus, Thomas
影响因子：
7.2
作者：
KRANZ, E;VONWIEGEN, P;LORZ, H
通讯作者：
LORZ, H
受精卵极化过程中细胞内动力学的活细胞成像
DOI：
--
发表时间：
2017
期刊：
影响因子：
--
作者：
Kimata;Y.;Ando;N.;Higaki;T.;Kurihara;D.;Kato;T.;Yamada;T.;Segami;S.;Morita;M. T.;Maeshima;M.;Hasezawa;S.;Higashiyama;T.;Tasaka;M.;Ueda;M.;植田美那子，木全祐資，栗原大輔，山田朋美，東山哲也;植田美那子;植田美那子;植田美那子
通讯作者：
植田美那子