Survival and regeneration of adult human photoreceptors in vitro

Survival and regeneration of adult human photoreceptors in vitro
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成人光感受器的体外存活和再生

DOI:
10.1016/0006-8993(94)90037-x
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发表时间:
1994
期刊:
影响因子:
2.9
通讯作者:
J. Sahel
J. Sahel
中科院分区:
医学3区
文献类型:
--
作者:
D. Hicks;V. Forster;H. Dreyfus;J. Sahel

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将来自摘除的眼组织的成人视网膜酶促解离并铺在塑料皿中用于在补充血清的培养基中进行细胞培养。在接种后的几天内,可以看到胶质样细胞的岛屿,圆形神经元生长在它们上面。对这些培养物的免疫细胞化学标记显示,几乎所有存活的神经元(> 98%)都是视杆细胞,并且它们在胶质细胞表面延伸长的神经突。因此,成年人的光感受器保留了显着的生存和再生能力,这样的准备可能是有价值的生理病理学和视网膜移植研究。
Adult human retina from enucleated ocular tissue was enzymatically dissociated and plated into plastic dishes for cell culture in serum-supplemented medium. Within a few days following seeding, islands of glial-like cells with rounded neurons growing on top of them were visible. Immunocytochemical labelling of these cultures revealed that virtually all the surviving neurons (> 98%) were rod photoreceptors, and that they extended long neurites across the glial cell surface. Hence, adult human photoreceptors retain a remarkable capacity for survival and regrowth, and such preparations may be of value for physiopathological and retinal grafting studies.
DOI: 10.1016/s0014-4827(85)80043-4
发表时间: 1985
影响因子: 3.7
作者:
Oka,MS;Frederick,JM;Landers,RA;Bridges,CD
通讯作者: Bridges,CD
DOI: 10.1016/0014-4835(88)90068-1
发表时间: 1988-12-01
影响因子: 3.4
作者:
LEWIS, GP;KASKA, DD;FISHER, SK
通讯作者: FISHER, SK
DOI: 10.1016/0014-4835(86)90017-5
发表时间: 1986-01-01
影响因子: 3.4
作者:
HICKS, D;MOLDAY, RS
通讯作者: MOLDAY, RS