Nanoparticulate cellular patches for cell-mediated tumoritropic delivery.
Nanoparticulate cellular patches for cell-mediated tumoritropic delivery.
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DOI：
10.1021/nn901319y
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发表时间：
2010-02-23
期刊：
影响因子：
17.1
通讯作者：
Anderson DG
中科院分区：
文献类型：
--
作者：
Cheng H;Kastrup CJ;Ramanathan R;Siegwart DJ;Ma M;Bogatyrev SR;Xu Q;Whitehead KA;Langer R;Anderson DG
靶向递送治疗药物到肿瘤仍然是癌症纳米医学的一个重要挑战。将纳米颗粒附着在具有致瘤性迁移特性的细胞上是解决这一挑战的一种有希望的方式。在这里，我们描述了一种技术，以创建纳米颗粒细胞贴片，保持附着在细胞膜长达2天。中性微球蛋白包被的纳米颗粒通过与生物素化的质膜结合而固定在细胞上。三维细胞外基质肿瘤模型显示，带有纳米颗粒贴片的人骨髓间充质干细胞保留了其固有的致瘤性（p值< 0.001）。此外，带有纳米颗粒贴片的人脐静脉内皮细胞能够保持其功能特性，并与未修饰的内皮细胞一样快速形成多细胞结构。这些结果提供了一种利用干细胞作为载体主动向肿瘤输送纳米结构和治疗方法的新策略，也表明纳米颗粒细胞贴片可能在组织再生中具有应用价值。
The targeted delivery of therapeutics to tumors remains an important challenge in cancer nanomedicine. Attaching nanoparticles to cells that have tumoritropic migratory properties is a promising modality to address this challenge. Here we describe a technique to create nanoparticulate cellular patches that remain attached to the membrane of cells for up to 2 days. Neutravidin-coated nanoparticles were anchored on cells by binding to biotinylated plasma membrane. Human bone marrow derived mesenchymal stem cells with nanoparticulate patches retained their inherent tumoritropic properties as shown using a tumor model in a 3D extracellular matrix (P-value < 0.001). Additionally, Human umbilical vein endothelial cells with nanoparticulate patches were able to retain their functional properties and form multicellular structures as rapidly as unmodified endothelial cells. These results provide a novel strategy to actively deliver nanostructures and therapeutics to tumors utilizing stem cells as carriers, and also suggest that nanoparticulate cellular patches may have applications in tissue regeneration.
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影响因子：
11.2
作者：
Nakamizo, A;Marini, F;Lang, FF
通讯作者：
Lang, FF
影响因子：
5.2
作者：
Lopez Ponte, Adriana;Marais, Emeline;Domenech, Jorge
通讯作者：
Domenech, Jorge
影响因子：
10.8
作者：
Choi, Mi-Ran;Stanton-Maxey, Katie J.;Clare, Susan E.
通讯作者：
Clare, Susan E.
影响因子：
15.9
作者：
Aboody, KS;Najbauer, J;Perides, G
通讯作者：
Perides, G
DOI：
10.1073/pnas.0601755103
发表时间：
2006-04-18
影响因子：
11.1
作者：
Farokhzad, OC;Cheng, JJ;Langer, R
通讯作者：
Langer, R