The impact of muscle disuse on muscle atrophy in severely burned rats.
The impact of muscle disuse on muscle atrophy in severely burned rats.
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DOI：
10.1016/j.jss.2010.08.032
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发表时间：
2010-12
期刊：
影响因子：
--
通讯作者：
Walters TJ
中科院分区：
文献类型：
--
作者：
Wu X;Baer LA;Wolf SE;Wade CE;Walters TJ
严重烧伤会导致持续的高代谢反应，从而导致肌肉质量的长期丧失和肌肉力量的下降。在这项研究中，我们试图利用严重皮肤烧伤和后肢卸载相结合的大鼠模型来确定肌肉停用是否对严重烧伤后的肌肉萎缩有额外的影响。SD大鼠40只，≈300g，随机分为假活动组(S/A组)、假后肢卸载组(S/HLU组)、烧伤活动组(B/A组)和烧伤后肢卸载组(B/HLU组)。大鼠造成40%全体表(TBSA)Ⅲ度烫伤，后肢卸载大鼠置于尾部牵引系统中。在第14天，用DEXA扫描测定瘦体重(LBM)，然后测量以快抽动为主的足底肌(PL)和以慢抽动为主的比目鱼肌(SL)的等长力学性质。测定肌肉重量(Wt)、蛋白质重量和湿/干重量。第14天，各治疗组体重均有明显下降，B/HLU组体重下降幅度明显大于B/A组、S/HLU组和S/A组，这可能与LBM的丢失有关。B/HLU组PL肌重量和Po最低(P<0.05)，与S/A、S/HLU或B/A组比较差异无统计学意义(P>0.05)。与S/A和B/A相比，S/HLU和B/HLU的SL肌重量和Po均显著减少，而S/HLU和B/HLU之间差异无统计学意义。皮肤灼伤和后肢卸载对肌肉萎缩有相加的影响，其特征是快(PL)和慢(SL)抽动肌的肌肉质量损失和肌力下降。在这个模型中，在这两种情况下，停用似乎是急性烧伤后持续肌肉萎缩的主要因素。
Severe burn induces a sustained hypermetabolic response, which causes long-term loss of muscle mass and decrease in muscle strength. In this study, we sought to determine whether muscle disuse has additional impact on muscle atrophy after severe burn using a rat model combining severe cutaneous burn and hindlimb unloading. Forty Sprague-Dawley rats (≈300g) were randomly assigned to sham ambulatory (S/A), sham hindlimb unloading (S/HLU), burn ambulatory (B/A) or burn hindlimb unloading (B/HLU) groups. Rats received a 40% total body surface (TBSA) full thickness scald burn, and rats with hindlimb unloading were placed in a tail traction system. At day 14, lean body mass (LBM) was determined using DEXA scan, followed by measurement of the isometric mechanical properties in the predominantly fast-twitch plantaris muscle (PL) and the predominantly slow-twitch soleus muscle (SL). Muscle weight (wt), protein wt, and wet/dry wt were determined. At day 14, body weight had decreased significantly in all treatment groups; B/HLU resulted in significantly greater loss compared to the B/A, S/HLU and S/A. The losses could be attributed to loss of LBM. PL muscle wt and Po were lowest in the B/HLU group (<0.05 vs. S/A, S/HLU or B/A). SL muscle wt and Po were significantly less in both S/HLU and B/HLU compared that of S/A and B/A; no significant difference was found between S/HLU and B/HLU. Cutaneous burn and hindlimb unloading have an additive effect on muscle atrophy, characterized by loss of muscle mass and decrease in muscle strength in both fast (PL) and slow (SL) twitch muscles. Of the two, disuse appeared to be the dominant factor for continuous muscle wasting after acute burn in this model.
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影响因子：
9.8
作者：
HASSELGREN, PO;JAMES, JH;FISCHER, JE
通讯作者：
FISCHER, JE
影响因子：
168.9
作者：
Low, JFA;Herndon, DN;Barrow, RE
通讯作者：
Barrow, RE
DOI：
10.1097/00005373-199704000-00009
发表时间：
1997-04-01
影响因子：
--
作者：
Matsuo, R;Herndon, DN;Suzuki, F
通讯作者：
Suzuki, F
影响因子：
5.8
作者：
Ferrando, AA;Stuart, CA;Wolfe, RR
通讯作者：
Wolfe, RR
影响因子：
2.2
作者：
HERNDON, DN;WILMORE, DW;MASON, AD
通讯作者：
MASON, AD