Pectic Bee Pollen Polysaccharide from Rosa rugosa Alleviates Diet-Induced Hepatic Steatosis and Insulin Resistance via Induction of AMPK/mTOR-Mediated Autophagy.
Pectic Bee Pollen Polysaccharide from Rosa rugosa Alleviates Diet-Induced Hepatic Steatosis and Insulin Resistance via Induction of AMPK/mTOR-Mediated Autophagy.
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DOI：
10.3390/molecules22050699
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发表时间：
2017-04-28
期刊：
影响因子：
--
通讯作者：
Zhou Y
中科院分区：
文献类型：
--
作者：
Li X;Gong H;Yang S;Yang L;Fan Y;Zhou Y
尽管它被用作治疗糖尿病和肥胖的营养药物，但蜂花粉的作用机制仍不清楚。从玫瑰花中分离得到果胶蜂花粉多糖（RBPP-P），并通过13 C-NMR和傅里叶变换红外光谱（FT-IR）对其结构进行表征。以高糖高脂饮食（high fat diet，HFD）诱导的肥胖小鼠和高糖高脂饮食诱导的HepG 2细胞为研究对象，通过自噬检测其对胰岛素功能和脂质代谢的影响。RBPP-P含有阿拉伯半乳聚糖、鼠李糖半乳糖醛酸聚糖I和同型半乳糖醛酸聚糖结构域。体内研究表明，RBPP-P能显著改善肥胖小鼠的胰岛素抵抗、葡萄糖耐受不良和肝脏脂肪变性。RBPP-P对肝脏脂肪变性和甘油三酯含量的抑制作用是通过增加肝脏中的自噬和脂肪酶表达来介导的。在AMPK敲减细胞（prkaa 1/2−/− MEF）和HFD喂养的小鼠组织（肝脏、性腺白色脂肪和腹股沟白色脂肪）中，RBPP-P以AMPK/mTOR依赖性方式增强肝脏中的自噬，但在脂肪组织中不增强。这些发现表明蜂花粉多糖通过AMPK/mTOR介导的信号通路促进自噬来减轻肝脏脂肪变性和胰岛素抵抗，表明RBPP-P可能是用于治疗肥胖和糖尿病的新型治疗剂。
Despite it is used as a nutraceutical against diabetes and obesity, the mechanism of action of bee pollen is still unclear. Pectic bee pollen polysaccharide (RBPP-P) was isolated from Rosa rugosa, and its structure was characterized by 13C-NMR and Fourier transform-infrared spectroscopy (FT-IR). Using high glucose and fatty acids-treated HepG2 cells and high fat diet (HFD)-induced obesity mice, we detected its effect on insulin function and lipid metabolism based on autophagy. RBPP-P contained arabinogalactan, rhamnogalacturonan I, and homogalacturonan domains. In vivo studies demonstrated that RBPP-P markedly ameliorated insulin resistance, glucose intolerance, and liver steatosis in obese mice. The suppressive effects of RBPP-P on liver steatosis and triglyceride content were mediated by increased autophagy and lipase expression in liver. In AMPK knockdown cells (prkaa 1/2−/− MEF) and HFD-fed mice tissues (liver, gonadal white adipose, and inguinal white adipose), RBPP-P enhanced autophagy in AMPK/mTOR-dependent way in liver, but not in adipose tissue. These findings demonstrated that bee pollen polysaccharide alleviated liver steatosis and insulin resistance by promoting autophagy via an AMPK/mTOR-mediated signaling pathway, suggesting that RBPP-P could be a novel therapeutic agent used for the treatment of obesity and diabetes.
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影响因子：
1.4
作者：
Tu, Yan;Zhang, Guo-Feng;Diao, Qi-Yu
通讯作者：
Diao, Qi-Yu
山楂中新型水溶性蜂花粉多糖的分离、纯化及其免疫生物学活性。
影响因子：
11.2
作者：
Li F;Yuan Q;Rashid F
通讯作者：
Rashid F
影响因子：
21.3
作者：
通讯作者：
--
影响因子：
7
作者：
Coppack, SW
通讯作者：
Coppack, SW
影响因子：
4.9
作者：
Latorre, J.;Moreno-Navarrete, J. M.;Ortega, F. J.
通讯作者：
Ortega, F. J.