Isolation, purification and immunobiological activity of a new water-soluble bee pollen polysaccharide from Crataegus pinnatifida Bge.
Isolation, purification and immunobiological activity of a new water-soluble bee pollen polysaccharide from Crataegus pinnatifida Bge.
复制标题
山楂中新型水溶性蜂花粉多糖的分离、纯化及其免疫生物学活性。
DOI：
10.1016/j.carbpol.2009.04.005
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发表时间：
2009-04-22
影响因子：
11.2
通讯作者：
Rashid F
中科院分区：
文献类型：
--
作者：
Li F;Yuan Q;Rashid F
从山楂蜂花粉中分离得到一种新的水溶性多糖。该多糖的两个组分，CPP-1和CPP-2，首先通过热水提取和纯化。CPP-1和CPP-2的平均分子量分别为3.7 × 105 Da和7.8 × 104 Da，通过气相色谱、傅立叶变换红外光谱、甲基化分析等手段对其结构进行了表征。采用巨噬细胞吞噬实验、自然杀伤细胞杀伤实验和脾淋巴细胞增殖实验评价CPP-1和CPP-2的作用。结果表明，CPP-1和CPP-2能显著提高小鼠腹腔巨噬细胞的吞噬率和吞噬指数。此外，这两个组分引起了显着的刺激大鼠脾细胞增殖。在50 μg/mL时，CPP-2比CPP-1更显著地激活NK细胞。这些结果表明，它们应该作为一种新的潜在的免疫刺激剂进行探索。
A novel water-soluble polysaccharide was obtained from bee pollen of Crataegus pinnatifida Bge. Two fractions of this polysaccharide, CPP-1 and CPP-2, were first extracted by hot-water and purified. The average molecular weight of CPP-1 and CPP-2 were approximately 3.7 × 105 Da and 7.8 × 104 Da, and their chemical structures were studied by gas chromatography (GC), Fourier transform-infrared (FT-IR) spectroscopy, methylation analysis. We evaluated the effects of CPP-1 and CPP-2 on the basis of phagocytosis of macrophage assay, natural killer cells cytotoxicity assay and spleen lymphocyte proliferation assay. The results showed CPP-1 and CPP-2 significantly induced phagocytic rates and phagocytic indexes by peritoneal macrophages. Moreover, these two fractions caused a significant stimulation of rat spleen cell proliferation. At 50 μg/mL, CPP-2 activated NK cells more significantly than CPP-1. These findings suggest that they should be explored as a novel potential immunostimulants.
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影响因子：
6.1
作者：
Bonvehí, JS;Torrentó, MS;Lorente, EC
通讯作者：
Lorente, EC
影响因子：
5.4
作者：
Kim, GY;Choi, GS;Park, YM
通讯作者：
Park, YM
影响因子：
6.5
作者：
Li, Xinjun;Nakagawa, Naoki;Sakurai, Naoki
通讯作者：
Sakurai, Naoki
影响因子：
11.2
作者：
Lim, JM;Joo, JH;Yun, JW
通讯作者：
Yun, JW
影响因子：
3.5
作者：
Chien, CA;Cheng, JL;Chen, ST
通讯作者：
Chen, ST