ENHANCING ctr1-10 ETHYLENE RESPONSE2 is a novel allele involved in CONSTITUTIVE TRIPLE-RESPONSE1-mediated ethylene receptor signaling in Arabidopsis.
ENHANCING ctr1-10 ETHYLENE RESPONSE2 is a novel allele involved in CONSTITUTIVE TRIPLE-RESPONSE1-mediated ethylene receptor signaling in Arabidopsis.
复制标题
增强 CTR1-10 乙烯响应 2 是拟南芥中参与组成型三重响应 1 介导的乙烯受体信号传导的新型等位基因
DOI：
10.1186/1471-2229-14-48
复制
发表时间：
2014-02-15
影响因子：
5.3
通讯作者：
Wen CK
中科院分区：
文献类型：
--
作者：
Xu A;Zhang W;Wen CK
背景：乙烯受体家族成员的信号输出通过未知的机制介导，激活Raf-like蛋白组成性三重应答1（CTR 1）负调节乙烯信号。乙烯受体组氨酸激酶（HK）结构域与CTR 1 N端之间的物理相互作用对于CTR 1介导的受体信号输出是必不可少的。为了推进我们的知识的参与CTR 1介导的乙烯受体信号，我们进行了基因筛选的突变，增强了组成性乙烯反应在弱ctr 1 -10 allele.Results：我们分离出一个功能丧失的等位基因增强ctr 1 -10乙烯反应2（ECR 2），并发现ecr 2 -1 ctr 1 -10和强等位基因ctr 1 -1赋予一个类似的，典型的组成性乙烯反应表型。遗传分析和转化研究表明，ECR 2作用于乙烯受体的下游和转录因子乙烯不敏感3（EIN 3）和EIN 3-LIKE 1（EIL 1）的上游，这些转录因子指导乙烯反应基因的表达。乙烯受体乙烯反应1（ETR 1）N末端的信号输出可由不依赖于CTR 1的途径介导。乙烯不敏感的etr 1 -1的N末端而不是全长同种型的表达拯救了ecr 2 -1 ctr 1 -10表型，这表明ECR 2参与了CTR 1介导的但不独立于CTR 1的乙烯受体信号传导。ECR 2定位于2号染色体上的着丝粒区域。由于不完整的序列和注释信息以及该区域罕见的染色体重组事件，ECR 2的克隆具有挑战性，并且仍在进行中。结论：ECR 2是一种新的参与由CTR 1介导的乙烯受体信号传导的等位基因。乙烯受体激活CTR 1可能需要ECR 2来抑制乙烯反应。
Background:The signal output of ethylene receptor family members is mediated by unknown mechanisms to activate the Raf-like protein CONSTITUTIVE TRIPLE RESPONSE1 (CTR1) in negatively regulating ethylene signaling. The physical interaction between the ethylene receptor histidine kinase (HK) domain and CTR1 N terminus is essential to the CTR1-mediated receptor signal output. To advance our knowledge of the involvement of CTR1-mediated ethylene receptor signaling, we performed a genetic screen for mutations that enhanced the constitutive ethylene response in the weak ctr1-10 allele.Results:We isolated a loss-of-function allele of ENHANCING ctr1-10 ETHYLENE RESPONSE2 (ECR2) and found that ecr2-1 ctr1-10 and the strong allele ctr1-1 conferred a similar, typical constitutive ethylene response phenotype. Genetic analyses and transformation studies suggested that ECR2 acts downstream of the ethylene receptors and upstream of the transcription factors ETHYLENE INSENSITIVE3 (EIN3) and EIN3-LIKE1 (EIL1), which direct the expression of ethylene response genes. Signal output by the N terminus of the ethylene receptor ETHYLENE RESPONSE1 (ETR1) can be mediated by a pathway independent of CTR1. Expression of the N terminus of the ethylene-insensitive etr1-1 but not the full-length isoform rescued the ecr2-1 ctr1-10 phenotype, which indicates the involvement of ECR2 in CTR1-mediated but not -independent, ethylene receptor signaling. ECR2 was mapped to the centromere region on chromosome 2. With incomplete sequence and annotation information and rare chromosome recombination events in this region, the cloning of ECR2 is challenging and still in progress.Conclusions:ECR2 is a novel allele involved in the ethylene receptor signaling that is mediated by CTR1. CTR1 activation by ethylene receptors may require ECR2 for suppressing the ethylene response.
登录
查看更多内容
影响因子：
2.9
作者：
Shakeel SN;Wang X;Binder BM;Schaller GE
通讯作者：
Schaller GE
影响因子：
14.9
作者：
Bugaut A;Balasubramanian S
通讯作者：
Balasubramanian S
影响因子：
7.2
作者：
Huang, YF;Li, H;Kieber, JJ
通讯作者：
Kieber, JJ
影响因子：
7.2
作者：
Clough, SJ;Bent, AF
通讯作者：
Bent, AF
DOI：
10.1126/science.1225974
发表时间：
2012-10-19
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Qiao H;Shen Z;Huang SS;Schmitz RJ;Urich MA;Briggs SP;Ecker JR
通讯作者：
Ecker JR