Two-photon imaging of neuronal activity in motor cortex of marmosets during upper-limb movement tasks.
Two-photon imaging of neuronal activity in motor cortex of marmosets during upper-limb movement tasks.
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DOI：
10.1038/s41467-018-04286-6
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发表时间：
2018-05-14
影响因子：
16.6
通讯作者：
Matsuzaki M
中科院分区：
文献类型：
--
作者：
Ebina T;Masamizu Y;Tanaka YR;Watakabe A;Hirakawa R;Hirayama Y;Hira R;Terada SI;Koketsu D;Hikosaka K;Mizukami H;Nambu A;Sasaki E;Yamamori T;Matsuzaki M
在行为动物的双光子成像揭示了与行为和认知功能相关的单细胞分辨率的神经元活动。然而，由于在运动任务训练方面的成功有限，绒猴提出了挑战。在这里，我们报告的协议，以训练头部固定的普通绒猴执行上肢运动任务，同时进行双光子成像的发展。经过2-5个月的训练，头部固定的绒猴可以控制一个机械手将光标移动到屏幕上的目标。我们进行双光子钙成像层2/3的运动皮层神经元在这个运动任务的性能，并检测任务相关的活动，从多个神经元在细胞和亚细胞的分辨率。在一个两个目标达成的任务，一些神经元显示方向选择活动在训练日。在短期的力场适应任务中，一些神经元改变其活动时，力场on. Two光子钙成像在行为的绒猴可能成为一个基本的技术，用于确定的空间组织的皮层动力学行为和认知。绒猴是神经科学中重要的模式生物，但在训练它们进行行为任务方面的成功有限。在这里，作者报告了他们在各种运动任务中训练绒猴的能力，并同时用双光子成像在运动皮层中成像神经动力学。
Two-photon imaging in behaving animals has revealed neuronal activities related to behavioral and cognitive function at single-cell resolution. However, marmosets have posed a challenge due to limited success in training on motor tasks. Here we report the development of protocols to train head-fixed common marmosets to perform upper-limb movement tasks and simultaneously perform two-photon imaging. After 2–5 months of training sessions, head-fixed marmosets can control a manipulandum to move a cursor to a target on a screen. We conduct two-photon calcium imaging of layer 2/3 neurons in the motor cortex during this motor task performance, and detect task-relevant activity from multiple neurons at cellular and subcellular resolutions. In a two-target reaching task, some neurons show direction-selective activity over the training days. In a short-term force-field adaptation task, some neurons change their activity when the force field is on. Two-photon calcium imaging in behaving marmosets may become a fundamental technique for determining the spatial organization of the cortical dynamics underlying action and cognition. Marmosets are an important model organism in neuroscience but there has only been limited success in training them on behavioral tasks. Here the authors report their ability to train marmosets in various motor tasks and simultaneously image neural dynamics in motor cortex with 2-photon imaging.
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影响因子：
25
作者：
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通讯作者：
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