Two VQ Proteins are Substrates of the OsMPKK6-OsMPK4 Cascade in Rice Defense Against Bacterial Blight.
Two VQ Proteins are Substrates of the OsMPKK6-OsMPK4 Cascade in Rice Defense Against Bacterial Blight.
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两种 VQ 蛋白是水稻防御细菌性枯萎病中 OsMPKK6-OsMPK4 级联的底物
DOI：
10.1186/s12284-021-00483-y
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发表时间：
2021-04-28
期刊：
影响因子：
--
通讯作者：
Wang S
中科院分区：
文献类型：
--
作者：
Li N;Yang Z;Li J;Xie W;Qin X;Kang Y;Zhang Q;Li X;Xiao J;Ma H;Wang S
背景植物特异性的缬氨酸-谷氨酰胺（valine-glutaminase，VQ）蛋白家族具有保守的FxxxVQxLTG结构域，在植物免疫中与丝裂原活化蛋白激酶（mitogen-activated protein kinase，MAPK）共同发挥功能。然而，VQ蛋白在MAPK介导的水稻抗病性中的作用在很大程度上仍是未知的。ResultsIn这项研究中，两个水稻VQ蛋白OsVQ 14和OsVQ 32被新鉴定为MAPK级联的信号传导组分，OsMPKK 6-OsMPK 4，调节水稻对水稻黄单胞菌的抗性。OsVQ 14和OsVQ 32均正调控水稻对Xoo的抗性。体外和体内研究表明，OsVQ 14和OsVQ 32与OsMPK 4物理相互作用并被其磷酸化。在过量表达OsMPKK 6的转基因植株中，OsMPK 4被高度磷酸化，表现出对Xoo的抗性增强。结论OsVQ 14和OsVQ 32是OsMPKK 6-OsMPK 4级联反应的底物，参与了水稻对Xoo的抗性增强，从而为水稻的诱导防御提供了一条更为完整的信号转导途径。
BackgroundThe plant-specific valine-glutamine (VQ) protein family with the conserved motif FxxxVQxLTG reportedly functions with the mitogen-activated protein kinase (MAPK) in plant immunity. However, the roles of VQ proteins in MAPK-mediated resistance to disease in rice remain largely unknown.ResultsIn this study, two rice VQ proteins OsVQ14 and OsVQ32 were newly identified to function as the signaling components of a MAPK cascade, OsMPKK6-OsMPK4, to regulate rice resistance toXanthomonas oryzaepv.oryzae(Xoo). BothOsVQ14andOsVQ32positively regulated rice resistance toXoo. In vitro and in vivo studies revealed that OsVQ14 and OsVQ32 physically interacted with and were phosphorylated by OsMPK4. OsMPK4 was highly phosphorylated in transgenic plants overexpressingOsMPKK6, which showed enhanced resistance toXoo. Meanwhile, phosphorylated OsVQ14 and OsVQ32 were also markedly accumulated inOsMPKK6-overexpressing transgenic plants.ConclusionsWe discovered that OsVQ14 and OsVQ32 functioned as substrates of the OsMPKK6-OsMPK4 cascade to enhance rice resistance toXoo, thereby defining a more complete signal transduction pathway for induced defenses.
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