Role of laminin in the attachment and metastasis of murine tumor cells.
Role of laminin in the attachment and metastasis of murine tumor cells.
复制标题
层粘连蛋白在小鼠肿瘤细胞附着和转移中的作用。
作者：
V. Terranova;L. Liotta;R. G. Russo;G. Martin
我们已经研究了两个小鼠转移性细胞系和一个转化的非转移性肉瘤细胞系对IV型（基底膜）胶原的附着。转移细胞优先附着于IV型胶原，而非转移细胞最好附着于I型胶原。层粘连蛋白增加的速度和数量的转移细胞附着到IV型胶原蛋白，而纤连蛋白没有影响。层粘连蛋白的抗体阻止了转移性细胞与IV型胶原的附着，而纤连蛋白的抗体阻止了非转移性细胞的附着。将细胞静脉内注射到C57 BL小鼠中后形成的肺转移的数量用于测量这些细胞系的转移倾向。通过在层粘连蛋白存在下附着于IV型胶原的能力选择的转移细胞亚群比未附着的细胞或与纤连蛋白附着的细胞产生更多的转移。此外，在注射到小鼠体内之前，将转移细胞与层粘连蛋白抗体一起孵育，显著减少了肺转移的数量。这些数据表明，层粘连蛋白在转移过程中促进转移性肿瘤细胞与基底膜的附着。
We have studied the attachment of two murine metastatic cell lines and of a transformed, nonmetastatic sarcoma cell line to type IV (basement membrane) collagen. The metastatic cells attached preferentially to type IV collagen, whereas the nonmetastic cells attached best to type I collagen. Laminin increased both the rate and the number of metastatic cells attaching to type IV collagen, while fibronectin had no effect. Antibodies to laminin prevented the attachment of metastatic cells to type IV collagen, while antibodies to fibronectin prevented the attachment of the nonmetastatic cells. The number of pulmonary metastases which formed after i.v. injection of cells into C57BL mice was used to measure the metastatic propensity of these cell lines. A subpopulation of the metastatic cells selected for by their ability to attach to type IV collagen in the presence of laminin produced more metastases than did unattached cells or cells attached with fibronectin. In addition, incubation of metastatic cells with antibody to laminin prior to injection into mice markedly reduced the number of lung metastases. These data suggest that laminin promotes the attachment of metastatic tumor cells to basement membrane during the metastaatic process.
纤连蛋白在转移性黑色素瘤细胞与内皮细胞及其基底层粘附中的作用。
影响因子：
3.7
作者：
Nicolson,GL;Irimura,T;Gonzalez,R;Ruoslahti,E
通讯作者：
Ruoslahti,E