Expression of UV-sensitive parapinopsin in the iguana parietal eyes and its implication in UV-sensitivity in vertebrate pineal-related organs.
Expression of UV-sensitive parapinopsin in the iguana parietal eyes and its implication in UV-sensitivity in vertebrate pineal-related organs.
复制标题
DOI：
10.1371/journal.pone.0039003
复制
发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Terakita A
中科院分区：
文献类型：
--
作者：
Wada S;Kawano-Yamashita E;Koyanagi M;Terakita A
低等脊椎动物的松果体相关器官具有区分不同波长的光的能力。这种波长区分是通过对UV或蓝光和可见光的对抗性光响应来实现的。在此之前，我们证明了，视蛋白的紫外线接收的七鳃鳗松果体器官和识别视蛋白基因的硬骨鱼和青蛙的松果体相关的器官据报道，歧视光。在这项研究中，我们报告的第一个确定的parapinopsin的爬行动物血统，并显示其在顶叶眼睛的绿色鬣蜥的表达。光谱分析表明，鬣蜥parapinopsin是一种紫外线敏感的色素，类似于七鳃鳗parapinopsin。有趣的是，免疫组织化学分析使用特异性抗体的parapinopsin和parietopsin，顶叶眼绿色敏感的色素，发现parapinopsin和parietopsin共定位在鬣蜥的顶叶眼感光细胞的外段。这些结果强烈表明，parainopsin的基础上的波长歧视涉及紫外线接收的鬣蜥顶叶的眼睛。目前的研究结果支持的想法，parainopsin是一种常见的色素潜在的紫外线敏感性的波长歧视的松果体相关的器官发现七鳃鳗爬行动物。
The pineal-related organs of lower vertebrates have the ability to discriminate different wavelengths of light. This wavelength discrimination is achieved through antagonistic light responses to UV or blue and visible light. Previously, we demonstrated that parapinopsin underlies the UV reception in the lamprey pineal organ and identified parapinopsin genes in teleosts and frogs of which the pineal-related organs were reported to discriminate light. In this study, we report the first identification of parapinopsin in the reptile lineage and show its expression in the parietal eye of the green iguana. Spectroscopic analysis revealed that iguana parapinopsin is a UV-sensitive pigment, similar to lamprey parapinopsin. Interestingly, immunohistochemical analyses using antibodies specific to parapinopsin and parietopsin, a parietal eye green-sensitive pigment, revealed that parapinopsin and parietopsin are colocalized in the outer segments of the parietal eye photoreceptor cells in iguanas. These results strongly suggest that parapinopsin underlies the wavelength discrimination involving UV reception in the iguana parietal eye. The current findings support the idea that parapinopsin is a common photopigment underlying the UV-sensitivity in wavelength discrimination of the pineal-related organs found from lampreys to reptiles.
登录
查看更多内容
DOI：
10.1016/s0169-328x(03)00059-7
发表时间：
2003-04-10
期刊：
MOLECULAR BRAIN RESEARCH
影响因子：
--
作者：
Moutsaki, P;Whitmore, D;Foster, RG
通讯作者：
Foster, RG
影响因子：
3.7
作者：
Kawano-Yamashita E;Koyanagi M;Shichida Y;Oishi T;Tamotsu S;Terakita A
通讯作者：
Terakita A
影响因子：
56.9
作者：
Su, CY;Luo, DG;Yau, KW
通讯作者：
Yau, KW
影响因子：
10.7
作者：
SAITOU, N;NEI, M
通讯作者：
NEI, M
影响因子：
4.5
作者：
UCHIDA, K;MORITA, Y
通讯作者：
MORITA, Y